Polydopamine Antioxidant Hydrogels for Wound Healing Applications.
Polydopamine Antioxidant Hydrogels for Wound Healing Applications.
复制标题
用于伤口愈合应用的聚多巴胺抗氧化剂水凝胶。
DOI：
10.3390/gels6040039
复制
发表时间：
2020-10-31
期刊：
影响因子：
--
通讯作者：
Peterson M
中科院分区：
文献类型：
--
作者：
O'Connor NA;Syed A;Wong M;Hicks J;Nunez G;Jitianu A;Siler Z;Peterson M
已知抗氧化剂可改善伤口愈合过程，并作为治疗慢性伤口的治疗策略进行研究。多巴胺是一种已知的具有抗氧化特性的神经递质，其可以聚合形成聚多巴胺（PDA）。在此，聚多巴胺被证明是一种抗氧化剂生物材料。在先前的工作中，我们开发了通过环氧氯丙烷和NaOH使多糖与多胺交联来制备水凝胶的方法。使用这种先前开发的方法，制备与聚多巴胺交联的葡聚糖水凝胶。凝胶的变暗表明水凝胶内聚多巴胺的掺入增加。除了碱性pH，聚多巴胺可以通过与聚乙烯亚胺（PEI）反应形成，这导致PEI-PDA共聚物。葡聚糖与PEI-PDA共聚物类似地交联，并产生更坚固、更深的凝胶，其具有更多的聚多巴胺掺入。水凝胶的形态和强度取决于多巴胺的进料比。含有聚多巴胺的水凝胶的抗氧化活性得到证实，并显示出依赖于水凝胶合成中使用的多巴胺的量。在2，2 ′-连氮双-（3-乙基苯并噻唑啉-6-磺酸）抗氧化剂测定中，多巴胺与葡聚糖进料比为0.5的水凝胶清除了78.8%的自由基，而不含多巴胺的凝胶仅清除了1.4%的自由基。离体伤口愈合测定显示含有PEI-PDA的水凝胶的相当大的细胞迁移。
Antioxidants are known to improve the wound healing process and are researched as a therapeutic strategy to treat chronic wounds. Dopamine is a known neurotransmitter with antioxidant properties that can be polymerized to form polydopamine (PDA). Herein, polydopamine is demonstrated as an antioxidant biomaterial. In prior work, we developed methodology to prepare hydrogels by crosslinking polysaccharides with polyamines via epichlorohydrin and NaOH. Using this previously developed methodology, dextran hydrogels crosslinked with polydopamine were prepared. Darkening of the gels indicated the increasing incorporation of polydopamine within the hydrogels. In addition to basic pH, polydopamine can be formed by reaction with polyethylene imine (PEI), which results in PEI-PDA copolymer. Dextran was similarly crosslinked with the PEI-PDA copolymer and resulted in sturdier, darker gels, which had more polydopamine incorporated. Hydrogel morphology and strength were dependent on the feed ratios of dopamine. Antioxidant activity of polydopamine containing hydrogel was confirmed and shown to be dependent on the amount of dopamine used in hydrogel synthesis. Hydrogels with 0.5 dopamine to dextran feed ratio scavenged 78.8% of radicals in a 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant assay while gels with no dopamine scavenged only 1.4% of radicals. An ex vivo wound healing assay showed considerable cell migration with the PEI-PDA containing hydrogel.
登录
查看更多内容
基于环糊精/共聚物包合物的原位电活性和抗氧化超分子水凝胶用于组织工程修复(a)
影响因子：
4.6
作者：
Cui, Haitao;Cui, Liguo;Chen, Xuesi
通讯作者：
Chen, Xuesi
影响因子：
11.2
作者：
Ahmed, Rifat Z.;Siddiqui, Khaizran;Ahmed, Nuzhat
通讯作者：
Ahmed, Nuzhat
影响因子：
2.4
作者：
Kim, Boram;Kang, Byungman;Kim, Ho-Joong
通讯作者：
Kim, Ho-Joong
DOI：
10.1016/j.ijbiomac.2014.08.003
发表时间：
2015-01
影响因子：
8.2
作者：
O'Connor NA;Abugharbieh A;Yasmeen F;Buabeng E;Mathew S;Samaroo D;Cheng HP
通讯作者：
Cheng HP
影响因子：
3.9
作者：
Liebscher, Juergen;Mrowczynski, Radoslaw;Beck, Sebastian
通讯作者：
Beck, Sebastian