Electrochemical L-lactic acid sensor based on immobilized ZnO nanorods with lactate oxidase.
Electrochemical L-lactic acid sensor based on immobilized ZnO nanorods with lactate oxidase.
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DOI：
10.3390/s120302456
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Willander M
中科院分区：
文献类型：
--
作者：
Ibupoto ZH;Shah SM;Khun K;Willander M
本文介绍了镀金玻璃基板的制备、氧化锌纳米棒的生长以及乳酸的电位响应。将乳酸氧化酶固定在氧化锌纳米棒上，结合戊二醛作为乳酸氧化酶的交联剂，制成了生物传感器。应用电位法测量了L-乳酸生物传感器的输出电势响应。我们注意到，该生物传感器在1×10−4~1×10 0 mm范围内具有较宽的线性检测范围，可接受的灵敏度为4 1.3 3±1.5 8 mV/cm2。此外，在抗坏血酸、尿素、葡萄糖、半乳糖、镁离子、钙离子等常见干扰物质的存在下，该传感器对L-乳酸具有良好的选择性，响应时间小于10 S。基于乳酸氧化酶固定的氧化锌纳米棒生物传感器的稳定性在三周以上。
In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10−4–1 × 100 mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards l-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.
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DOI：
10.3390/bios1040153
发表时间：
2011-10-27
期刊：
Biosensors
影响因子：
--
作者：
Ibupoto ZH;Ali SM;Khun K;Chey CO;Nur O;Willander M
通讯作者：
Willander M
影响因子：
12.6
作者：
Hirano, K;Yamato, H;Ohwa, M
通讯作者：
Ohwa, M
影响因子：
13.1
作者：
Kerman, Kagan;Saito, Masato;Tamiya, Eiichi
通讯作者：
Tamiya, Eiichi
影响因子：
4
作者：
Hsueh, Ting-Jen;Chang, Shoou-Jinn;Chen, I.-Cherng
通讯作者：
Chen, I.-Cherng
DOI：
10.3390/s91108911
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Fulati A;Ali SM;Riaz M;Amin G;Nur O;Willander M
通讯作者：
Willander M