A Point Mutation V419L in the Sodium Channel Gene from Natural Populations of Aedes aegypti Is Involved in Resistance to λ-Cyhalothrin in Colombia.
A Point Mutation V419L in the Sodium Channel Gene from Natural Populations of Aedes aegypti Is Involved in Resistance to λ-Cyhalothrin in Colombia.
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DOI：
10.3390/insects9010023
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发表时间：
2018-02-14
期刊：
影响因子：
3
通讯作者：
Triana-Chavez O
中科院分区：
文献类型：
--
作者：
Granada Y;Mejía-Jaramillo AM;Strode C;Triana-Chavez O
蚊子对拟除虫菊酯的抗性主要是由被称为击倒抗性(Kdr)的靶点不敏感引起的。在这项工作中，我们检测了来自哥伦比亚三个城市的埃及伊蚊钠通道基因I、II、III和IV区域的部分点突变。对抗药性蚊子钠通道基因的部分编码区域进行了测序，并使用基于简单等位基因特异性聚合酶链式反应(AS-PCR)的方法分析了种群水平的突变。此前报道的V1016I和F1534C突变在这三个城市被发现，频率分别为0.04到0.41和0.56到0.71。此外，还发现了Ae基因第419位密码子(V419L)的新突变。来自贝洛市、里奥哈查市和维拉维森西奥市的埃及人的等位基因频率分别为0.06、0.36和0.46。有趣的是，药敏试验表明，来自贝洛的蚊子对λ-氯氟氰菊酯拟除虫菊酯敏感，而来自里奥哈查和维拉维森西奥的蚊子则具有抗药性。在Riohacha和Villavicencio，V419L和V1016I突变与λ-氯氟氰菊酯耐药性呈正相关。F1534C在这三个种群中的频率很高，这表明该突变可能导致对λ-氯氟氰菊酯以外的杀虫剂产生抗药性，特别是I型拟除虫菊酯。需要进一步的研究来证实这一假设。
Resistance to pyrethroids in mosquitoes is mainly caused by target site insensitivity known as knockdown resistance (kdr). In this work, we examined the point mutations present in portions of domains I, II, III, and IV of the sodium channel gene in Aedes aegypti mosquitoes from three Colombian municipalities. A partial region coding for the sodium channel gene from resistant mosquitoes was sequenced, and a simple allele-specific PCR-based assay (AS-PCR) was used to analyze mutations at the population level. The previously reported mutations, V1016I and F1534C, were found with frequencies ranging from 0.04 to 0.41, and 0.56 to 0.71, respectively, in the three cities. Moreover, a novel mutation, at 419 codon (V419L), was found in Ae. aegypti populations from Bello, Riohacha and Villavicencio cities with allelic frequencies of 0.06, 0.36, and 0.46, respectively. Interestingly, the insecticide susceptibility assays showed that mosquitoes from Bello were susceptible to λ-cyhalothrin pyrethroid whilst those from Riohacha and Villavicencio were resistant. A positive association between V419L and V1016I mutations with λ-cyhalothrin resistance was established in Riohacha and Villavicencio. The frequency of the F1534C was high in the three populations, suggesting that this mutation could be conferring resistance to insecticides other than λ-cyhalothrin, particularly type I pyrethroids. Further studies are required to confirm this hypothesis.
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