NONINVASIVE SAMPLING FOR DETECTION OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS AND GENOMIC DNA IN ASIAN (ELEPHAS MAXIMUS) AND AFRICAN (LOXODONTA AFRICANA) ELEPHANTS.

NONINVASIVE SAMPLING FOR DETECTION OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS AND GENOMIC DNA IN ASIAN (ELEPHAS MAXIMUS) AND AFRICAN (LOXODONTA AFRICANA) ELEPHANTS.
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DOI:
10.1638/2019-0112
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发表时间:
2020-06
期刊:
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians
影响因子:
--
通讯作者:
Gilardi K
Gilardi K
中科院分区:
其他
文献类型:
--
作者:
Jeffrey A;Evans TS;Molter C;Howard LL;Ling P;Goldstein T;Gilardi K

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大象亲内皮疱疹病毒(EEHV)出血性疾病(EEHV-HD)威胁着亚洲象(Elephas maximus)在北美的种群可持续性。非洲象(Loxodonta africana)也报告了集群病例。对分布国家大象种群的风险尚不清楚。目前,EEHV的检测依赖于采样大象训练侵入性血液和树干清洗收集。为了评估非侵入性样本采集的选择,配对侵入性收集(血液,树干清洗和口腔拭子),和非侵入性收集(咀嚼植物和粪便)样本进行了比较,超过6周,从9个亚洲象和12个非洲象。同时检测到EEHV脱落的一对象鼻清洗和粪便样本从一个非洲象。在从4头亚洲象采集的6份咀嚼植物样品中鉴定出大象γ疱疹病毒-1散毒。非侵入性收集的样本可用于检测大象疱疹病毒脱落。需要更长的采样周期来评估EEHV检测的无创采样的临床有用性。
Elephant endotheliotropic herpesvirus (EEHV) hemorrhagic disease (EEHV-HD) threatens Asian elephant (Elephas maximus) population sustainability in North America. Clusters of cases have also been reported in African elephants (Loxodonta africana). Risk to range country elephant populations is unknown. Currently, EEHV detection depends upon sampling elephants trained for invasive blood and trunk wash collection. To evaluate noninvasive sample collection options, paired invasively collected (blood, trunk wash and oral swabs), and noninvasively collected (chewed plant and fecal) samples were compared over 6 wk from 9 Asian elephants and 12 African elephants. EEHV shedding was detected simultaneously in a paired trunk wash and fecal sample from one African elephant. Elephant γ herpesvirus-1 shedding was identified in six chewed plant samples collected from four Asian elephants. Noninvasively collected samples can be used to detect elephant herpesvirus shedding. Longer sampling periods are needed to evaluate the clinical usefulness of noninvasive sampling for EEHV detection.
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