Development of the conditionally immortalized testicular Sertoli cell line TTE3 expressing Sertoli cell specific genes from mice transgenic for temperature sensitive simian virus 40 large T antigen gene.
Development of the conditionally immortalized testicular Sertoli cell line TTE3 expressing Sertoli cell specific genes from mice transgenic for temperature sensitive simian virus 40 large T antigen gene.
复制标题
条件永生化睾丸支持细胞系 TTE3 的开发，该细胞系表达来自温度敏感猿病毒 40 大 T 抗原基因转基因小鼠的支持细胞特异性基因。
DOI：
10.1016/s0022-5347(05)65359-1
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
S. Asano
中科院分区：
文献类型：
--
作者：
Y. Tabuchi;S. Ohta;N. Yanai;M. Obinata;T. Kondo;H. Fuse;S. Asano
目的从携带温度敏感猴病毒40大T抗原基因pSVtsA58的转基因小鼠中建立条件永生化的睾丸支持细胞系，并对其进行鉴定。材料与方法8周龄雄性转基因小鼠建立的细胞在允许(33℃)或不允许(39℃)的温度下培养在I型胶原预包被的培养容器中。用逆转录聚合酶链式反应、免疫细胞化学和Western印迹分析支持细胞特异性蛋白的表达。大T抗原仅在33℃时的细胞核中表达，表明细胞的温敏性生长表型是温度敏感型猴病毒40大T抗原作用的结果。该细胞在软琼脂中未显示任何集落形成活性，在裸鼠皮下组织中未形成肿瘤，表明TTE3细胞未转化。细胞表达编码钢铁因子、抑制素-α、转铁蛋白、卵泡刺激素受体和硫酸糖蛋白-2的信使RNA。此外，波形蛋白和闭合带-1的表达分别位于细胞质和细胞边界。有趣的是，在39℃时，转铁蛋白和闭锁带-1的表达水平明显升高。结论具有这些独特特性的TTE3细胞可以作为研究支持细胞功能调节的有用模型。
PurposeWe developed and characterized a conditionally immortalized testicular Sertoli cell line from transgenic mice bearing the temperature sensitive simian virus 40 large T antigen gene pSVtsA58.Materials and MethodsEstablished cells from 8-week-old male transgenic mice were cultured at a permissive (33C) or nonpermissive (39C) temperature on a collagen type I pre-coated culture vessel. The expression of Sertoli cell specific proteins was analyzed by reverse transcriptase-polymerase chain reaction, immunocytochemical testing and Western blot analysis.ResultsThe Sertoli cell line TTE3 grew at 33C but not at 39C. Large T antigen was expressed only in the nuclei at 33C, indicating that the temperature sensitive growth phenotype of the cells arose as a result of the function of temperature sensitive simian virus 40 large T antigen. The cells did not show any colony forming activity in soft agar or form tumors in subcutaneous tissue in nude mice, showing that TTE3 cells were not transformed. The cells expressed messenger RNAs encoding steel factor, inhibin-α, transferrin, follicle-stimulating hormone receptor and sulfated glycoprotein-2. Moreover, expression of vimentin and zonula occludens-1 was observed in the cytoplasm and on the boundaries of the cells, respectively. Interestingly expression levels of transferrin and zonula occludens-1 were significantly elevated at 39C.ConclusionsTTE3 cells with these unique characteristics should serve as a useful model to study the regulation of Sertoli cell function.
登录
查看更多内容
青春期前大鼠支持细胞系 93RS2 的发育和表征。
DOI：
--
发表时间：
1997
期刊：
Journal of andrology.
影响因子：
--
作者：
Jiang,C;Hall,SJ;Boekelheide,K
通讯作者：
Boekelheide,K
影响因子：
4.8
作者：
C. T. Anthony;M. Rosselli;M. Skinner
通讯作者：
C. T. Anthony;M. Rosselli;M. Skinner
DOI：
10.1016/s0021-9258(18)43419-9
发表时间：
1980-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Skinner;M. Griswold
通讯作者：
M. Skinner;M. Griswold
影响因子：
3.7
作者：
HOFMANN, MC;NARISAWA, S;MILLAN, JL
通讯作者：
MILLAN, JL
影响因子：
3.6
作者：
R. Vernon;T. Lane;J. Angello;H. Sage
通讯作者：
R. Vernon;T. Lane;J. Angello;H. Sage