Immune cell census in murine atherosclerosis: cytometry by time of flight illuminates vascular myeloid cell diversity.
Immune cell census in murine atherosclerosis: cytometry by time of flight illuminates vascular myeloid cell diversity.
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DOI：
10.1093/cvr/cvy109
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发表时间：
2018-08-01
影响因子：
10.8
通讯作者：
Monaco C
中科院分区：
文献类型：
--
作者：
Cole JE;Park I;Ahern DJ;Kassiteridi C;Danso Abeam D;Goddard ME;Green P;Maffia P;Monaco C
动脉粥样硬化的特征是在疾病的早期阶段开始大量骨髓细胞浸润。骨髓细胞是动脉粥样硬化形成期间血管免疫的关键参与者。然而，由于在血管组织中共有的标记物表达和非典型异质性，血管髓样细胞的亚群难以分辨。我们应用了高维度的质谱细胞术研究动脉粥样硬化中的髓系细胞亚群。给载脂蛋白E缺陷（ApoE−/−）小鼠喂食普通饲料或高脂肪（西方）饮食12周。使用飞行时间流式细胞术（CyTOF），用一组35种金属偶联抗体探测单细胞主动脉制备物。来自ApoE−/−小鼠腹主动脉的活CD 45+细胞上的标记物表达聚类鉴定了13个广泛的白细胞群体。单核细胞、巨噬细胞、1型和2型常规树突状细胞（cDC 1和cDC 2）、浆细胞样树突状细胞（pDC）、中性粒细胞、嗜酸性粒细胞、B细胞、CD 4+和CD 8 + T细胞、γδ T细胞、自然杀伤（NK）细胞和先天性淋巴细胞（ILC）群体约占活CD 45+主动脉细胞的95%。应用于Lin-CD 11blo-hi细胞的自动聚类算法揭示了20个髓样细胞簇。食物和高脂肪喂养的动物之间的比较揭示了单核细胞（Ly 6C+和Ly 6C-）、pDC和CD 11 c+巨噬细胞亚群随着高脂肪喂养而增加。同时，巨噬细胞的CD 206 + CD 169+亚群的比例显著降低，cDC 2也是如此。基于CyTOF的ApoE−/−小鼠动脉粥样硬化斑块内免疫细胞亚群的综合图谱为血管隔室内的髓样细胞识别提供了工具，它揭示了高脂肪喂养使髓样细胞库偏向炎症单核细胞-巨噬细胞群体，而不是动脉粥样硬化形成期间的常驻巨噬细胞表型和cDC 2。
Atherosclerosis is characterized by the abundant infiltration of myeloid cells starting at early stages of disease. Myeloid cells are key players in vascular immunity during atherogenesis. However, the subsets of vascular myeloid cells have eluded resolution due to shared marker expression and atypical heterogeneity in vascular tissues. We applied the high-dimensionality of mass cytometry to the study of myeloid cell subsets in atherosclerosis. Apolipoprotein E-deficient (ApoE−/−) mice were fed a chow or a high fat (western) diet for 12 weeks. Single-cell aortic preparations were probed with a panel of 35 metal-conjugated antibodies using cytometry by time of flight (CyTOF). Clustering of marker expression on live CD45+ cells from the aortas of ApoE−/− mice identified 13 broad populations of leucocytes. Monocyte, macrophage, type 1 and type 2 conventional dendritic cell (cDC1 and cDC2), plasmacytoid dendritic cell (pDC), neutrophil, eosinophil, B cell, CD4+ and CD8+ T cell, γδ T cell, natural killer (NK) cell, and innate lymphoid cell (ILC) populations accounted for approximately 95% of the live CD45+ aortic cells. Automated clustering algorithms applied to the Lin-CD11blo-hi cells revealed 20 clusters of myeloid cells. Comparison between chow and high fat fed animals revealed increases in monocytes (both Ly6C+ and Ly6C−), pDC, and a CD11c+ macrophage subset with high fat feeding. Concomitantly, the proportions of CD206+ CD169+ subsets of macrophages were significantly reduced as were cDC2. A CyTOF-based comprehensive mapping of the immune cell subsets within atherosclerotic aortas from ApoE−/− mice offers tools for myeloid cell discrimination within the vascular compartment and it reveals that high fat feeding skews the myeloid cell repertoire toward inflammatory monocyte-macrophage populations rather than resident macrophage phenotypes and cDC2 during atherogenesis.
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影响因子：
64.5
作者：
Levine JH;Simonds EF;Bendall SC;Davis KL;Amir el-AD;Tadmor MD;Litvin O;Fienberg HG;Jager A;Zunder ER;Finck R;Gedman AL;Radtke I;Downing JR;Pe'er D;Nolan GP
通讯作者：
Nolan GP
影响因子：
16.8
作者：
Bendall SC;Nolan GP;Roederer M;Chattopadhyay PK
通讯作者：
Chattopadhyay PK
随着年龄的增长，逐步替代了胚胎来源的心脏巨噬细胞。
DOI：
10.1084/jem.20140639
发表时间：
2014-10-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Molawi K;Wolf Y;Kandalla PK;Favret J;Hagemeyer N;Frenzel K;Pinto AR;Klapproth K;Henri S;Malissen B;Rodewald HR;Rosenthal NA;Bajenoff M;Prinz M;Jung S;Sieweke MH
通讯作者：
Sieweke MH
影响因子：
--
作者：
Cho, Kae Won;Morris, David L.;Lumeng, Carey N.
通讯作者：
Lumeng, Carey N.
影响因子：
4.3
作者：
Chen H;Lau MC;Wong MT;Newell EW;Poidinger M;Chen J
通讯作者：
Chen J