Biocompatibility and in vivo degradation of chitosan based hydrogels as potential drug carrier
Biocompatibility and in vivo degradation of chitosan based hydrogels as potential drug carrier
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壳聚糖基水凝胶作为潜在药物载体的生物相容性和体内降解
DOI：
10.1080/09205063.2017.1412244
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Yangsheng Chen
中科院分区：
文献类型：
--
作者：
Feng Su;Yu;ou Wang;Xue Liu;Xin Shen;Xingjian Zhang;Quansheng Xing;Lihong Wang;Yangsheng Chen
摘要以1-（3-二甲氨基丙基）-3-乙基碳二亚胺盐酸盐/N-羟基琥珀酰亚胺（EDC/NHS）为交联剂和催化剂，在室温下制备了羧甲基壳聚糖接枝聚乳酸（CMCS-PLA）和羧甲基壳聚糖（CMCS）水凝胶。的水凝胶的生物相容性进行了评价，目的是评估其作为药物载体的潜力。考虑了生物相容性的各个方面，包括MTT试验、琼脂扩散试验、乳酸脱氢酶（LDH）释放、溶血试验、血浆凝固时间（PRT）和动态凝血时间。MTT法显示两种水凝胶对L-929细胞的细胞毒性为0或1。CMCS和CMCS-PLA的LDH释放分别为26%和29%，略高于阴性对照（21%），远低于阴性对照（87%）。CMCS和CMCS-PLA的溶血率分别为1.4%和1.7%，表明两种材料具有优异的抗溶血性能。CMCS和CMCS-PLA的PRT值比阳性对照值高77%和99%。结果表明，该水凝胶具有良好的体外细胞相容性和血液相容性。通过在大鼠背部皮下注射评价了体内降解和组织相容性。体内19天后，CMCS和CMCS-PLA水凝胶完全降解，水凝胶周围的炎症反应也完全消失。因此，由CMCS和CMCS-PLA形成的水凝胶具有优异的生物相容性，是有希望作为潜在的药物载体。
Abstract Carboxymethyl chitosan-graft-polylactide (CMCS-PLA) and carboxymethyl chitosan (CMCS) hydrogels were prepared by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) as crosslinking agent and catalyst at room temperature. The biocompatibility of the hydrogels was evaluated with the aim of assessing their potential as drug carrier. Various aspects of biocompatibility were considered, including MTT assay, agar diffusion test, release of lactate dehydrogenase (LDH), hemolytic test, plasma recalcification time (PRT), and dynamic clotting time. MTT assay showed that the cytotoxicity level of both hydrogels to L-929 cells was 0 or 1. The LDH release of CMCS and CMCS-PLA was 26 and 29%, respectively, which is slightly higher than that of the negative control (21%) and much lower than that of the negative control (87%). The hemolysis ratio of CMCS and CMCS-PLA was 1.4 and 1.7%, respectively, suggesting outstanding anti-hemolysis properties of both materials. The PRT value of CMCS and CMCS-PLA was higher by 77 and 99% than the value of the positive control. All the results revealed that the hydrogels present good cytocompatibility and hemocompatibility in vitro. In vivo degradation and tissue compatibility were evaluated by subcutaneous injection in the dorsal area of rats. CMCS and CMCS-PLA hydrogels were completely degraded and the inflammatory response also completely disappeared around hydrogels after 19 days in vivo. It is thus concluded that hydrogels formed of CMCS and CMCS-PLA with outstanding biocompatibility are promising as potential drug carrier.
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影响因子：
2.5
作者：
Liu Shuilian;Zhou Yang;C. Fuhua;Zhu Shoujin;S. Feng;L. Suming
通讯作者：
Liu Shuilian;Zhou Yang;C. Fuhua;Zhu Shoujin;S. Feng;L. Suming
影响因子：
5
作者：
McGinley, Emma Louise;Coleman, David C.;Fleming, Garry J. P.
通讯作者：
Fleming, Garry J. P.
影响因子：
5.9
作者：
Qasim, Saad B.;Husain, Shehriar;Rehman, Ihtesham Ur
通讯作者：
Rehman, Ihtesham Ur
DOI：
10.1080/09205063.2013.814096
发表时间：
2013-10
期刊：
Journal of Biomaterials Science, Polymer Edition
影响因子：
--
作者：
Lingling Tian;M. Prabhakaran;X. Ding;S. Ramakrishna
通讯作者：
Lingling Tian;M. Prabhakaran;X. Ding;S. Ramakrishna
DOI：
10.1007/s10856-010-4070-0
发表时间：
2010-07-01
影响因子：
3.7
作者：
Zhang, Erlin;Chen, Haiyan;Shen, Feng
通讯作者：
Shen, Feng