Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals.
Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals.
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DOI：
10.1126/sciadv.adg3918
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发表时间：
2023-04-21
期刊：
影响因子：
13.6
通讯作者：
Aharoni, Daniel
中科院分区：
文献类型：
--
作者：
Guo, Changliang;Blair, Garrett J.;Sehgal, Megha;Jimka, Federico N. Sangiuliano;Bellafard, Arash;Silva, Alcino J.;Golshani, Peyman;Basso, Michele A.;Blair, Hugh Tad;Aharoni, Daniel
在比小鼠大的自由行为动物中成像大群体，单细胞荧光动力学仍然是神经科学的关键奋进。我们提出了一种大视场开源微型显微镜（MiniLFOV），设计用于自由行为大鼠的大尺度（3.6 mm × 2.7 mm），细胞分辨率神经成像。它具有高达3.5 mm ± 100 μm的电动可调工作距离，集成了绝对头部方向传感器，MiniLFOV能够进行脑深部和皮层成像，并已在自由行为大鼠中通过同时成像海马CA 1层中>1000个GCaMP 7表达神经元进行验证，并在头部固定小鼠中通过同时成像~ 2000个神经元在背侧皮层通过颅窗。MiniLFOV还支持使用新型无线数据采集扩展板的可选无线操作。我们希望UCLA Miniscope平台的这种新的开源实现将使研究人员能够解决有关自由行为动物大脑功能的新假设。一个大视野的开源微型显微镜能够对大鼠和小鼠的大量神经元进行成像。
Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present a large-field-of-view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 mm × 2.7 mm), cellular resolution neural imaging in freely behaving rats. It has an electrically adjustable working distance of up to 3.5 mm ± 100 μm, incorporates an absolute head orientation sensor, and weighs only 13.9 g. The MiniLFOV is capable of both deep brain and cortical imaging and has been validated in freely behaving rats by simultaneously imaging >1000 GCaMP7s-expressing neurons in the hippocampal CA1 layer and in head-fixed mice by simultaneously imaging ~2000 neurons in the dorsal cortex through a cranial window. The MiniLFOV also supports optional wire-free operation using a novel, wire-free data acquisition expansion board. We expect that this new open-source implementation of the UCLA Miniscope platform will enable researchers to address novel hypotheses concerning brain function in freely behaving animals. A large field of view open-source miniature microscope is capable of imaging large populations of neurons in rats and mice.
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影响因子：
16.6
作者：
Frank AC;Huang S;Zhou M;Gdalyahu A;Kastellakis G;Silva TK;Lu E;Wen X;Poirazi P;Trachtenberg JT;Silva AJ
通讯作者：
Silva AJ
影响因子：
25
作者：
Dombeck, Daniel A.;Harvey, Christopher D.;Tian, Lin;Looger, Loren L.;Tank, David W.
通讯作者：
Tank, David W.
DOI：
10.1126/science.abg1278
发表时间：
2021-07-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dotson NM;Yartsev MM
通讯作者：
Yartsev MM
影响因子：
5.3
作者：
Hart, Evan E.;Blair, Garrett J.;Izquierdo, Alicia
通讯作者：
Izquierdo, Alicia
影响因子：
64.5
作者：
Kingsbury, Lyle;Huang, Shan;Hong, Weizhe
通讯作者：
Hong, Weizhe