Transmission of West Nile virus by Culex quinquefasciatus say infected with Culex Flavivirus Izabal.
Transmission of West Nile virus by Culex quinquefasciatus say infected with Culex Flavivirus Izabal.
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DOI：
10.1371/journal.pntd.0000671
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发表时间：
2010-05-04
影响因子：
3.8
通讯作者：
Miller BR
中科院分区：
文献类型：
--
作者：
Kent RJ;Crabtree MB;Miller BR
蚊子特异性黄病毒的自然历史及其对西尼罗病毒传播效率的潜在影响尚不清楚。本研究的目的是确定先前感染黄库蚊病毒（CxFV）是否会改变Cx载体的能力。致倦库蚊：从危地马拉传播西尼罗河病毒（WNV）共循环毒株。对经胸内接种致倦库蚊和阴性致倦库蚊分别给予西尼罗河病毒感染血餐。在西尼罗河病毒暴露两周后，分别用斑块滴定法在蚊子个体体、腿或唾液的Vero细胞上测量西尼罗河病毒的感染、传播和传播。Cx的附加基团。分别用WNV单独或WNV+CxFV Izabal同时胸内接种致倦库蚊，接种后第9天采集唾液。西尼罗河病毒在白纹伊蚊C6/36细胞和Cx细胞中的生长。致倦库蚊未被既往感染的Izabal病毒所抑制。Cx的载体能力差异无统计学意义。跨多个西尼罗河病毒血滴度和两个Cx菌落对未感染西尼罗河病毒或感染西尼罗河病毒的致倦库蚊进行西尼罗河病毒检测。紫光p > 0.05)。然而，更多的Cx。同时接种两种病毒的洪都拉斯致倦库蚊比单独接种西尼罗河病毒的致倦库蚊传播率高（p = 0.0014）。联合接种传播西尼罗河病毒的蚊子唾液中也含有CxFV，而单独接种CxFV的蚊子唾液中不含病毒。在序贯感染实验中，既往感染CxFV Izabal对西尼罗河病毒的复制、感染、传播或Cx传播没有显著影响。然而，当蚊子同时接种两种病毒时，洪都拉斯殖民地的西尼罗河病毒传播增强。与大多数已知的黄病毒（黄病毒科：黄病毒属）不同，只感染昆虫的黄病毒是一种独特的黄病毒，只感染无脊椎动物。近年来，由于从世界各地的多种蚊子物种中发现和鉴定了许多新的黄病毒，对昆虫黄病毒的研究有所增加。这些病毒的广泛发现引发了有关黄病毒进化和这些病毒对诸如西尼罗河病毒等具有公共卫生重要性的黄病毒传播的潜在影响的问题。因此，我们测试了从危地马拉致倦库蚊中分离出的一种仅限昆虫的黄病毒（CxFV Izabal）对从同一种蚊子和地点同时分离的一株西尼罗河病毒的生长和传播的影响。既往感染C6/36（白纹伊蚊）细胞或Cx；携带CxFV Izabal的致倦库蚊没有改变西尼罗河病毒的复制动力学，也没有显著影响两种不同种群的西尼罗河病毒感染、传播或传播率，这些蚊子被喂食含有不同浓度西尼罗河病毒的血餐。这些数据表明，CxFV在本质上可能对西尼罗河病毒的传播效率没有显著影响。
The natural history and potential impact of mosquito-specific flaviviruses on the transmission efficiency of West Nile virus (WNV) is unknown. The objective of this study was to determine whether or not prior infection with Culex flavivirus (CxFV) Izabal altered the vector competence of Cx. quinquefasciatus Say for transmission of a co-circulating strain of West Nile virus (WNV) from Guatemala. CxFV-negative Culex quinquefasciatus and those infected with CxFV Izabal by intrathoracic inoculation were administered WNV-infectious blood meals. Infection, dissemination, and transmission of WNV were measured by plaque titration on Vero cells of individual mosquito bodies, legs, or saliva, respectively, two weeks following WNV exposure. Additional groups of Cx. quinquefasciatus were intrathoracically inoculated with WNV alone or WNV+CxFV Izabal simultaneously, and saliva collected nine days post inoculation. Growth of WNV in Aedes albopictus C6/36 cells or Cx. quinquefasciatus was not inhibited by prior infection with CxFV Izabal. There was no significant difference in the vector competence of Cx. quinquefasciatus for WNV between mosquitoes uninfected or infected with CxFV Izabal across multiple WNV blood meal titers and two colonies of Cx. quinquefasciatus (p>0.05). However, significantly more Cx. quinquefasciatus from Honduras that were co-inoculated simultaneously with both viruses transmitted WNV than those inoculated with WNV alone (p = 0.0014). Co-inoculated mosquitoes that transmitted WNV also contained CxFV in their saliva, whereas mosquitoes inoculated with CxFV alone did not contain virus in their saliva. In the sequential infection experiments, prior infection with CxFV Izabal had no significant impact on WNV replication, infection, dissemination, or transmission by Cx. quinquefasciatus, however WNV transmission was enhanced in the Honduras colony when mosquitoes were inoculated simultaneously with both viruses. Unlike most known flaviviruses (Family, Flaviviridae: Genus, Flavivirus), insect-only flaviviruses are a unique group of flaviviruses that only infect invertebrates. The study of insect-only flaviviruses has increased in recent years due to the discovery and characterization of numerous novel flaviviruses from a diversity of mosquito species around the world. The widespread discovery of these viruses has prompted questions regarding flavivirus evolution and the potential impact of these viruses on the transmission of flaviviruses of public health importance such as WNV. Therefore, we tested the effect of Culex flavivirus Izabal (CxFV Izabal), an insect-only flavivirus isolated from Culex quinquefasciatus mosquitoes in Guatemala, on the growth and transmission of a strain of WNV isolated concurrently from the same mosquito species and location. Prior infection of C6/36 (Aedes albopictus mosquito) cells or Cx. quinquefasciatus with CxFV Izabal did not alter the replication kinetics of WNV, nor did it significantly affect WNV infection, dissemination, or transmission rates in two different colonies of mosquitoes that were fed blood meals containing varying concentrations of WNV. These data demonstrate that CxFV probably does not have a significant effect on WNV transmission efficiency in nature.
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影响因子：
11.4
作者：
Elbashir, SM;Martinez, J;Tuschl, T
通讯作者：
Tuschl, T
影响因子：
3.7
作者：
Kim, Dal Y.;Guzman, Hilda;Tesh, Robert B.
通讯作者：
Tesh, Robert B.
影响因子：
4.2
作者：
Campbell CL;Keene KM;Brackney DE;Olson KE;Blair CD;Wilusz J;Foy BD
通讯作者：
Foy BD
DOI：
10.4269/ajtmh.2009.80.85
发表时间：
2009-01-01
影响因子：
3.3
作者：
Farfan-Ale, Jose A.;Lorono-Pino, Maria A.;Blitvich, Bradley J.
通讯作者：
Blitvich, Bradley J.
影响因子：
2.7
作者：
Crabtree, MB;Sang, RC;Miller, BR
通讯作者：
Miller, BR