Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification.
Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification.
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DOI：
10.3390/s18010207
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发表时间：
2018-01-12
期刊：
影响因子：
--
通讯作者：
Kim DH
中科院分区：
文献类型：
--
作者：
Cho IH;Lee J;Kim J;Kang MS;Paik JK;Ku S;Cho HM;Irudayaraj J;Kim DH
电化学免疫传感器采用抗体作为捕获和检测手段，以产生用于目标分子定量分析的电荷。这种传感器类型可用作用于检测护理点测试（POCT）的小型化装置。实现关于灵敏度的高性能分析一直是开发这种类型的生物传感器系统的关键问题之一。许多现代纳米技术的努力允许开发具有高灵敏度的创新电化学生物传感器，通过采用各种纳米材料，结合表面改性和生物共轭技术，促进电子转移和信号示踪剂的承载能力。在这篇综述中，我们介绍了新的纳米材料（例如，碳纳米管、石墨烯、铟锡氧化物、纳米线和金属纳米颗粒）以构建高性能电极。此外，我们描述了如何通过采用纳米材料作为载体，并使聚合酶复合物与氧化还原循环信号放大相关联，以增加信号示踪剂的数量。每种方法的优点和缺点在整个审查中得到考虑。我们期望这些综述的信号增强策略将应用于下一版本的侧流式纸色谱和微流体免疫传感器，它们被认为是最实用的POCT生物传感器平台。
An electrochemical immunosensor employs antibodies as capture and detection means to produce electrical charges for the quantitative analysis of target molecules. This sensor type can be utilized as a miniaturized device for the detection of point-of-care testing (POCT). Achieving high-performance analysis regarding sensitivity has been one of the key issues with developing this type of biosensor system. Many modern nanotechnology efforts allowed for the development of innovative electrochemical biosensors with high sensitivity by employing various nanomaterials that facilitate the electron transfer and carrying capacity of signal tracers in combination with surface modification and bioconjugation techniques. In this review, we introduce novel nanomaterials (e.g., carbon nanotube, graphene, indium tin oxide, nanowire and metallic nanoparticles) in order to construct a high-performance electrode. Also, we describe how to increase the number of signal tracers by employing nanomaterials as carriers and making the polymeric enzyme complex associated with redox cycling for signal amplification. The pros and cons of each method are considered throughout this review. We expect that these reviewed strategies for signal enhancement will be applied to the next versions of lateral-flow paper chromatography and microfluidic immunosensor, which are considered the most practical POCT biosensor platforms.
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影响因子：
7.4
作者：
Fanjul-Bolado, Pablo;Hernandez-Santos, David;Costa-Garcia, Agustin
通讯作者：
Costa-Garcia, Agustin
DOI：
10.3390/s17010054
发表时间：
2016-12-29
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Choi YB;Jeon WY;Kim HH
通讯作者：
Kim HH
影响因子：
9.3
作者：
Bosker WM;Huestis MA
通讯作者：
Huestis MA
影响因子：
12.6
作者：
Arya, Sunil K.;Dey, Abhishek;Bhansali, Shekhar
通讯作者：
Bhansali, Shekhar
影响因子：
12.6
作者：
Fähnrich, KA;Pravda, M;Guilbault, GG
通讯作者：
Guilbault, GG