Temporal characterization of homology-independent centromere coupling in meiotic prophase.
Temporal characterization of homology-independent centromere coupling in meiotic prophase.
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DOI：
10.1371/journal.pone.0010336
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发表时间：
2010-04-23
期刊：
影响因子：
3.7
通讯作者：
Dawson DS
中科院分区：
文献类型：
--
作者：
Obeso D;Dawson DS
在过去的30年里，一些关于非同源着丝粒在减数分裂前期配对或联合的报道出现在文献中。最近，在芽殖酵母中也报道了着丝粒的非同源性成对缔合，称为着丝粒偶联。在同源染色体排列的过程中，着丝粒会与非同源的伴侣配对，这似乎是自相矛盾的，然而在广泛的物种中这种现象的保守性表明它可能在减数分裂中发挥重要作用。为了更好地定义这种现象在芽殖酵母中的作用，进行了实验以将着丝粒偶联置于里程碑减数分裂事件的背景下。在减数分裂程序开始后不久，发现着丝粒从单个簇重新组织成非同源对。着丝粒偶联检测染色体复制完成，并持续而重组蛋白Dmc 1加载到染色体上，表明着丝粒偶联持续通过双链断裂形成的时间。在没有联会复合体的组成部分，Zip 1，着丝粒耦合是无法检测到的，在所有的时间检查，证实了这个蛋白质在这个过程中的重要作用。最后，着丝粒偶联的及时释放取决于重组起始酶Spo 11，这表明同源配对/重组中的事件与着丝粒偶联的调节之间存在联系。根据我们的研究结果，我们提出了一个作用，着丝粒耦合在阻断同源着丝粒之间的相互作用，重组启动正在发生。
Over the past thirty years several reports of the pairing or association of non-homologous centromeres during meiotic prophase have appeared in the literature. Recently, the homology-independent pairwise association of centromeres, termed centromere coupling, was also reported in budding yeast. It seems paradoxical that centromeres would pair with non-homologous partners during a process intended to align homologous chromosomes, yet the conservation of this phenomenon across a wide range of species suggests it may play an important role in meiosis. To better define the role of this phenomenon in budding yeast, experiments were preformed to place centromere coupling within the context of landmark meiotic events. Soon after the initiation of the meiotic program, centromeres were found to re-organize from a single cluster into non-homologous couples. Centromere coupling is detected as soon as chromosome replication is finished and persists while the recombination protein Dmc1 is loaded onto the chromosomes, suggesting that centromere coupling persists through the time of double strand break formation. In the absence of the synaptonemal complex component, Zip1, centromere coupling was undetectable, at all times examined, confirming the essential role of this protein on this process. Finally, the timely release of centromere coupling depends on the recombination-initiating enzyme, Spo11, suggesting a connection between events in homologous pairing/recombination and the regulation of centromere coupling. Based on our results we propose a role for centromere coupling in blocking interactions between homologous centromeres as recombination initiation is taking place.
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