A Changeable Lab-on-a-Chip Detector for Marine Nonindigenous Microorganisms in Ship's Ballast Water.
A Changeable Lab-on-a-Chip Detector for Marine Nonindigenous Microorganisms in Ship's Ballast Water.
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一种可更换的芯片实验室检测器，用于检测船舶压载水中的海洋非本地微生物
DOI：
10.3390/mi9010020
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发表时间：
2018-01-05
期刊：
影响因子：
3.4
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Maw MM;Pan X;Peng Z;Wang Y;Zhao L;Dai B;Wang J
船舶压载水中的外来物种在世界范围内的扩散和入侵，已经对生态、经济和人类健康造成了危害和威胁。船舶压载水中海洋入侵物种的快速、准确检测至关重要。本文采用可更换的微流控芯片检测器对船舶压载水水质进行分析，以满足船舶压载水管理和沉积物公约D-2标准的要求。将微流控芯片技术、阻抗脉冲传感技术和LED光诱导叶绿素荧光（LED-LICF）检测技术相结合，设计了检测系统。该系统可以测量目标微生物的数量、大小、形状和体积，还可以确定叶绿素荧光强度，这是分析浮游植物活动的重要因素。目标样品是小球藻、杜氏盐藻、亚心形扁藻、藻类、大肠杆菌和肠球菌。整个检测或操作可在几分钟内完成，只需使用微米体积的样品溶液即可在线检测。有效数据输出同时显示在阻抗脉冲幅度和荧光强度信号方面。该检测系统通过改变微流控芯片上微通道的尺寸，实现了多尺寸的真实的实时检测。由于该系统能够成功地检测无标记微生物，因此只要对系统进行一些修改，该系统就可以适用于原位检测。
The spread and invasion of many nonindigenous species in the ship’s ballast water around the world has been a hazard and threat to ecology, economy, and human health. The rapid and accurate detection of marine invasive species in ship’s ballast water is essential. This article is aimed at analysing ballast water quality by means of a changeable microfluidic chip detector thus comply with the D-2 standard of ship’s ballast water management and sediment convention. The detection system was designed through the integration of microfluidic chip technology, the impedance pulse sensing and LED light induced chlorophyll fluorescence (LED-LICF) detection. This system can measure the number, size, shape, and volume of targeted microorganisms, and it can also determine the chlorophyll fluorescence intensity, which is an important factor in analysing the activity of phytoplankton. The targeted samples were Chlorella volutis, Dunaliella salina, Platymonas subcordiformis, Chrysophytes, Escherichia coli, and Enterococci. The whole detection or operation can be accomplished through online detection in a few minutes with using micron volume of the sample solution. The valid data outputs are simultaneously displayed in terms of both impedance pulse amplitudes and fluorescent intensity signals. The detection system is designed for multi-sizes real time detection through changing the microchannel sizes on the microfluidic chip. Because it can successfully detect the label-free microorganisms, the system can be applicable to in-situ detections with some modifications to the system.
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DOI：
10.3390/s90604483
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Heo J;Hua SZ
通讯作者：
Hua SZ
影响因子：
3.9
作者：
Anderson, Will;Lane, Rebecca;Trau, Matt
通讯作者：
Trau, Matt
影响因子：
3.3
作者：
Han, Sun-Young;Kim, Bo-Ra;Kim, Baek-Il
通讯作者：
Kim, Baek-Il
影响因子：
3.7
作者：
Stone, HA;Kim, S
通讯作者：
Kim, S
集成水下微流体装置中微藻细胞尺寸谱的检测
DOI：
10.1016/j.jembe.2015.08.016
发表时间：
2015-12-01
影响因子：
2
作者：
Wang, Junsheng;Song, Younan;Li, Dongqing
通讯作者：
Li, Dongqing