A high-throughput device for size based separation of C. elegans developmental stages.
A high-throughput device for size based separation of C. elegans developmental stages.
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DOI：
10.1039/c3lc51334c
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发表时间：
2014-05-21
期刊：
影响因子：
6.1
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Ai X;Zhuo W;Liang Q;McGrath PT;Lu H
秀丽隐杆线虫是一种广泛应用于研究发育、衰老和行为的模式生物。这些生物学研究中的许多需要对大量蠕虫进行分期以测定动物的同步种群。传统的同步技术，如人工采摘，重力分层和化学漂白是劳动密集型的，可能会扰乱动物的生理。因此，需要一种简单廉价的技术来基于蠕虫的发育阶段以最小的扰动分选蠕虫的混合种群。在这里，我们展示了一种简单但准确和高通量的技术，根据动物的大小进行排序，这与发育阶段密切相关。该装置由一系列几何优化的支柱组成，这些支柱充当筛子，允许特定大小的蠕虫快速通过。通过优化的腔室高度、柱间距和驱动压力，这些二元分离装置能够在两个不同阶段独立分离蠕虫混合物，平均效率约为95%，每分钟的吞吐量为数百个蠕虫。此外，当顺序使用四个设备时，我们证明了以>85%的总效率对所有发育阶段的蠕虫混合物进行分层的能力。
Caenorhabditis elegans is a widely used model organism to study development, aging and behavior. Many of these biological studies require staging a large number of worms to assay a synchronized population of animals. Conventional synchronization techniques such as manual picking, gravity stratification and chemical bleaching are labor-intensive and could perturb animals’ physiology. Thus, there is a need for a simple inexpensive technology to sort a mixed population of worms based on their developmental stages with minimal perturbation. Here we demonstrate a simple but accurate and high-throughput technique to sort based on animal size, which correlates well with developmental stages. The device consists of an array of geometrically optimized pillars that act as a sieve to allow worms of specific sizes to rapidly move through. With optimized chamber heights, pillar spacing and driving pressures, these binary separation devices are capable of independently separating a mixture of worms at two different stages at average efficiency of around 95%, and throughput of hundreds of worms per minute. In addition, when four devices are used sequentially, we demonstrate the ability to stratify a mixture of worms of all developmental stages with >85% overall efficiency.
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影响因子：
64.5
作者：
BARGMANN, CI;HARTWIEG, E;HORVITZ, HR
通讯作者：
HORVITZ, HR
影响因子：
6.1
作者：
Han, Bicheng;Kim, Daeyeon;Shin, Jennifer H.
通讯作者：
Shin, Jennifer H.
影响因子：
6.1
作者：
Chung K;Zhan M;Srinivasan J;Sternberg PW;Gong E;Schroeder FC;Lu H
通讯作者：
Lu H
影响因子：
56.9
作者：
CHALFIE, M;TU, Y;PRASHER, DC
通讯作者：
PRASHER, DC
影响因子：
64.5
作者：
ELLIS, HM;HORVITZ, HR
通讯作者：
HORVITZ, HR