IL-1B(3954) polymorphism and red complex bacteria increase IL-1β (GCF) levels in periodontitis.
IL-1B(3954) polymorphism and red complex bacteria increase IL-1β (GCF) levels in periodontitis.
复制标题
IL-1B（3954）多态性和红色复合菌增加牙周炎患者IL-1β（GCF）水平。
DOI：
10.1111/jre.12850
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发表时间：
2021-06
影响因子：
3.5
通讯作者：
Papathanasiou E
中科院分区：
文献类型：
--
作者：
Pani P;Tsilioni I;McGlennen R;Brown CA;Hawley CE;Theoharides TC;Papathanasiou E
本研究的目的是比较IL-1B（3954）-SNP阳性和IL-1B（3954）-SNP阴性牙周炎患者健康和牙周炎部位龈沟液（GCF）中IL-1β水平及其与细菌谱的关系。牙周炎的易感性与几个危险因素有关，包括多个基因位点的等位基因变异。IL-1基因簇的变异与牙周炎风险增加有关。IL-1B（3954）-SNP先前已与慢性牙周炎患者GCF或牙周组织中IL-1β水平升高以及特定牙周病原体水平升高相关。没有足够的证据表明IL-1B基因多态性是否通过最终调节GCF中IL-1β的水平、龈下微生物谱或两者来影响牙周炎的易感性。从32名牙周炎患者中收集GCF、龈下菌斑和颊上皮细胞。通过酶联免疫吸附试验（ELISA）测量GCF IL-1β水平。细菌菌斑样本进行了分析，11牙周病原体使用聚合酶链反应（PCR）分析与16 SrRNA基因的每种细菌的特异性引物。通过鉴定多态性T等位基因的携带者来确定IL-1B（3954）-SNP状态。IL-1B（3954）-SNP与IL-1β GCF水平（量和浓度）之间显示出显著相关性。同时存在两种或三种红色复合菌与牙周炎部位IL-1β GCF水平升高相关（部位水平分析）。同时存在所有三种红色牙周复合病原体和IL-1B（3954）-SNP与牙周炎部位的最高IL-1β GCF水平相关。我们的研究结果表明，IL-1B（3954）-SNP和红色复合菌种类与牙周炎部位GCF中IL-1β水平升高独立相关。更好地了解遗传学，细菌和炎症之间的相互作用对于开发更有效的牙周炎诊断，预后和治疗工具至关重要。
The aim of this study was to compare IL-1β levels in gingival crevicular fluid (GCF) from healthy and periodontitis sites of IL-1B(3954)-Single Nucleotide Polymorphism (SNP) positive and IL-1B(3954)-SNP negative periodontitis subjects in association with their bacterial profiles. Susceptibility to periodontitis has been associated with several risk factors, including allelic variants at multiple gene loci. Variations in the IL-1 gene cluster have been linked with increased risk for periodontitis. IL-1B(3954)-SNP has been previously associated with increased levels of IL-1β in GCF or periodontal tissues in chronic periodontitis patients, as well as higher levels of specific periodontal pathogens. There is insufficient evidence to conclude if IL-1B gene polymorphisms affect the susceptibility to periodontitis by ultimately modulating the levels of IL-1β in GCF, the subgingival microbial profile or both. GCF, subgingival plaque, and buccal epithelial cells were collected from 32 individuals with periodontitis. GCF IL-1β levels were measured by an enzyme-linked immunosorbent assay (ELISA). Bacterial plaque samples were analyzed for 11 periodontal pathogens using polymerase chain reaction (PCR) analysis with specific primers for the 16SrRNA gene of each bacterium. IL-1B(3954)-SNP status was determined by identifying the carriers of the polymorphic T allele. A significant association was shown between IL-1B(3954)-SNP and IL-1β GCF levels (amount and concentration). The concomitant presence of two or three red complex bacterial species was associated with increased IL-1β GCF levels in periodontitis sites (site-level analysis). The concurrent presence of all three red complex periodontal pathogens and IL-1B(3954)-SNP was associated with the highest IL-1β GCF levels in periodontitis sites. Our results indicate an independent association of both IL-1B(3954)-SNP and red complex bacterial species with increased IL-1β levels in GCF of periodontitis sites. A better understanding of the interaction between genetics, bacteria, and inflammation is essential to develop more effective diagnostic, prognostic, and therapeutic tools for periodontitis.
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