An anisotropic nanofiber/microsphere composite with controlled release of biomolecules for fibrous tissue engineering.
An anisotropic nanofiber/microsphere composite with controlled release of biomolecules for fibrous tissue engineering.
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DOI：
10.1016/j.biomaterials.2010.01.098
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发表时间：
2010-05
期刊：
影响因子：
14
通讯作者：
Mauck, Robert L.
中科院分区：
文献类型：
--
作者：
Ionescu, Lara C.;Lee, Gregory C.;Sennett, Brian J.;Burdick, Jason A.;Mauck, Robert L.
取向纳米纤维支架能够重现肌肉骨骼系统中纤维增强组织的结构层次。虽然这些电纺纤维支架在接种细胞时能提供可引导组织形成的物理信号，但在不破坏支架机械性能的情况下化学引导细胞群的能力将提高此类构建体的成熟度，并在体外和体内为该系统增加额外的功能。在这项研究中，我们开发了一种制造技术，将载药微球包埋在纳米纤维支架内。我们假设，将微球包埋在纤维之间相较于将微球置于纤维内部对机械性能的不良影响更小，并且该复合物将表现出多种模型化合物的持续释放。我们的结果表明，直径在10 - 20微米的微球能够以剂量依赖的方式进行电纺以形成纳米纤维复合物。当通过牺牲性聚氧化乙烯（PEO）纤维群递送时，在去除牺牲性递送成分后，微球仍能牢固地包埋在缓慢降解的聚己内酯（PCL）纤维之间。对于微球包埋在每根纤维内的复合物，其刚度和模量随着微球密度的增加而降低，而当微球包埋在纤维之间时，刚度没有变化。复合结构的释放曲线与游离微球相似，最初有一个突释，随后模型分子持续释放超过4周。此外，多种模型分子从单个支架复合物中释放出来，证明了这些结构具有理想的多因子控制释放能力，适用于复杂生长因子的递送。
Aligned nanofibrous scaffolds can recapitulate the structural hierarchy of fiber-reinforced tissues of the musculoskeletal system. While these electrospun fibrous scaffolds provide physical cues that can direct tissue formation when seeded with cells, the ability to chemically guide a population of cells, without disrupting scaffold mechanical properties, would improve the maturation of such constructs and add additional functionality to the system both in vitro and in vivo. In this study, we developed a fabrication technique to entrap drug-delivering microspheres within nanofibrous scaffolds. We hypothesized that entrapping microspheres between fibers would have a less adverse impact on mechanical properties than placing microspheres within the fibers themselves, and that the composite would exhibit sustained release of multiple model compounds. Our results show that microspheres ranging from 10~20 microns in diameter could be electrospun in a dose-dependent manner to form nanofibrous composites. When delivered in a sacrificial PEO fiber population, microspheres remained securely entrapped between slow-degrading PCL fibers after removal of the sacrificial delivery component. Stiffness and modulus of the composite decreased with increasing microsphere density for composites in which microspheres were entrapped within each fiber, while stiffness did not change when microspheres were entrapped between fibers. The release profiles of the composite structures were similar to free microspheres, with an initial burst release followed by a sustained release of the model molecules over 4 weeks. Further, multiple model molecules were released from a single scaffold composite, demonstrating the capacity for multi-factor controlled release ideal for complex growth factor delivery from these structures.
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DOI：
10.1002/jbm.b.30510
发表时间：
2006-10-01
影响因子：
3.4
作者：
Jiang, Hongliang;Hu, Yingqian;Zhu, Kangjie
通讯作者：
Zhu, Kangjie
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
10.8
作者：
Kenawy, ER;Bowlin, GL;Wnek, GE
通讯作者：
Wnek, GE
影响因子：
14
作者：
Lee, Wonjae;Wiseman, Meredith E.;Frank, Curtis W.
通讯作者：
Frank, Curtis W.
影响因子：
6.2
作者：
Hong, Yi;Fujimoto, Kazuro;Wagner, William R.
通讯作者：
Wagner, William R.