Repositioning Titanium: An In Vitro Evaluation of Laser-Generated Microporous, Microrough Titanium Templates As a Potential Bridging Interface for Enhanced Osseointegration and Durability of Implants.
Repositioning Titanium: An In Vitro Evaluation of Laser-Generated Microporous, Microrough Titanium Templates As a Potential Bridging Interface for Enhanced Osseointegration and Durability of Implants.
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DOI：
10.3389/fbioe.2017.00077
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发表时间：
2017
影响因子：
5.7
通讯作者：
Oreffo ROC
中科院分区：
文献类型：
--
作者：
Tang D;Yang LY;Ou KL;Oreffo ROC
尽管钛合金仍然是当今制造生物医学植入物的首选生物材料，但由于骨整合不足，此类设备可能会在植入后 15 年内失效。此外，已发现合金金属离子释放引起的磨损碎片毒性会引起副作用，包括神经毒性和慢性炎症。钛具有众所周知的生物相容性、耐腐蚀性和高弹性模量，如果以表面支架或桥接装置的形式利用，可以解决这些问题。采用一种新颖的三维培养方法来研究激光生成的微孔、微粗糙医用 IV 级钛模板对人类骨骼干细胞 (SSC) 的潜在骨诱导和骨整合能力。尽管在基础条件下倒置培养超过 21 天，但接种在环氧乙烷灭菌模板的粗糙 90 µm 孔表面上的人类 SSC 仍具有很强的粘附性，并显示出早期成骨分化。模板表面上有限的细胞迁移凸显了高表面润湿性在最大化细胞粘附、扩散和细胞-生物材料相互作用方面的重要性，而锥形孔内有限的细胞向内生长则强调了孔几何形状和尺寸在确定植入装置骨整合程度方面的关键作用。总体研究结果表明，对孔隙率和表面润湿性进行适当优化的纯钛器械仍可以在提高未来植入技术的长期功效、耐用性和安全性方面发挥重要作用。
Although titanium alloys remain the preferred biomaterials for the manufacture of biomedical implants today, such devices can fail within 15 years of implantation due to inadequate osseointegration. Furthermore, wear debris toxicity due to alloy metal ion release has been found to cause side-effects including neurotoxicity and chronic inflammation. Titanium, with its known biocompatibility, corrosion resistance, and high elastic modulus, could if harnessed in the form of a superficial scaffold or bridging device, resolve such issues. A novel three-dimensional culture approach was used to investigate the potential osteoinductive and osseointegrative capabilities of a laser-generated microporous, microrough medical grade IV titanium template on human skeletal stem cells (SSCs). Human SSCs seeded on a rough 90-µm pore surface of ethylene oxide-sterilized templates were observed to be strongly adherent, and to display early osteogenic differentiation, despite their inverted culture in basal conditions over 21 days. Limited cellular migration across the template surface highlighted the importance of high surface wettability in maximizing cell adhesion, spreading and cell-biomaterial interaction, while restricted cell ingrowth within the conical-shaped pores underlined the crucial role of pore geometry and size in determining the extent of osseointegration of an implant device. The overall findings indicate that titanium only devices, with appropriate optimizations to porosity and surface wettability, could yet play a major role in improving the long-term efficacy, durability, and safety of future implant technology.
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影响因子：
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作者：
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