Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus
Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus
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Wnt 信号通路通过刺参的进化模式和基因表达与肠道再生相关
DOI：
10.3389/fgene.2019.00112
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发表时间：
2019-02
影响因子：
3.7
通讯作者：
Jianhai Xiang
中科院分区：
文献类型：
--
作者：
Jianbo Yuan;Yi Gao;Lina Sun;Songjun Jin;Xiaojun Zhang;Chengzhang Liu;Fuhua Li;Jianhai Xiang
许多棘皮动物都是再生物种，表现出非凡的再生能力，海参是一种代表性的生物，可以在内脏切除后再生整个肠道。有许多信号通路参与肠再生过程，但目前尚不清楚哪一条通路是肠再生所必需的。在本研究中，我们对这些再生相关的信号通路进行了全基因组的综合分析，发现Wnt信号通路是再生物种中最保守的信号通路之一。另外，在这些信号通路中，我们发现Wnt信号通路是唯一一条在再生棘皮动物中受到正选择的信号通路，也是唯一一条在肠再生过程中被差异表达基因富集的信号通路。因此，Wnt信号通路的编码序列和基因表达均受到自然选择的影响，为肠道再生提供了遗传结构; Wnt 7、Fz 7和Dv是3个正选择基因，也是Wnt信号通路的3个上游基因。它们在再生的早期阶段都显著上调，这可能显著有助于Wnt信号的早期激活和肠再生的启动。通过RNA干扰敲低Wnt 7和Dv 1的表达显著抑制肠的延伸，这意味着它们是肠再生所必需的。作为再生相关的重要基因，其下游基因Mycis在整个再生过程中均保持高度表达，这可能使Wnt/c-Myc信号通路成为促进肠再生的重要途径。因此，我们有理由认为Wnt信号通路在海参肠再生过程中起着重要作用，甚至在其他棘皮动物的肠再生过程中也起着重要作用。
Many echinoderms are regenerative species that exhibit exceptional regenerative capacity, and sea cucumber is a representative organism that could regenerate the whole intestine after evisceration. There are many signaling pathways participate in the regeneration process, but it is not clear which is essential for the intestinal regeneration. In this study, we performed genome-wide comprehensive analyses on these regeneration-related signaling pathways, and found the Wnt signaling pathway was one of the most conservative pathways among regenerative species. Additionally, among these signaling pathways, we found that the Wnt signaling pathway was the only one under positive selection in regenerative echinoderms, and the only one enriched by differentially expressed genes during the intestinal regeneration. Thus, it suggests both coding sequence and gene expression of the Wnt signaling pathway have been shaped by natural selection to provide the genetic architecture for intestinal regeneration.Wnt7, Fz7, andDvlare the three positively selected genes and also happen to be three upstream genes in the Wnt signaling pathway. They are all significantly upregulated at the early stages of regeneration, which may contribute significantly to the early activation of Wnt signaling and the initiation of intestinal regeneration. Expression knockdown ofWnt7andDvlby RNA interference significantly inhibit intestinal extension, implying that they are essential for intestinal regeneration. As an important regeneration-related gene, the downstream genec-Mycis also conserved and highly expressed during the whole regeneration stages, which may make the Wnt/c-Myc signaling to be an important way to promote intestinal regeneration. Therefore, it is reasonable to conclude that the Wnt signaling pathway is the chosen one to play an important role in intestinal regeneration of sea cucumbers, or even in the regeneration of other echinoderms.
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影响因子：
11.5
作者：
Evangelista, Marie;Tian, Hua;de Sauvage, Frederic J.
通讯作者：
de Sauvage, Frederic J.
影响因子：
2.5
作者：
T. Shibata;T. Oji;K. Akasaka;K. Agata
通讯作者：
T. Shibata;T. Oji;K. Akasaka;K. Agata
Hippo 通路在器官大小控制、组织稳态和癌症中的作用
影响因子：
64.5
作者：
Yu FX;Zhao B;Guan KL
通讯作者：
Guan KL
影响因子：
4.4
作者：
Ortiz-Pineda PA;Ramírez-Gómez F;Pérez-Ortiz J;González-Díaz S;Santiago-De Jesús F;Hernández-Pasos J;Del Valle-Avila C;Rojas-Cartagena C;Suárez-Castillo EC;Tossas K;Méndez-Merced AT;Roig-López JL;Ortiz-Zuazaga H;García-Arrarás JE
通讯作者：
García-Arrarás JE
DOI：
10.4238/2013.november.7.7
发表时间：
2013-11
期刊：
Genetics and molecular research : GMR
影响因子：
--
作者：
Lina Sun;Hongsheng Yang;Muyan Chen;D. Xu
通讯作者：
Lina Sun;Hongsheng Yang;Muyan Chen;D. Xu