Prevalence of autoantibodies that bind to kidney tissues in cats and association risk with antibodies to feline viral rhinotracheitis, calicivirus, and panleukopenia.
Prevalence of autoantibodies that bind to kidney tissues in cats and association risk with antibodies to feline viral rhinotracheitis, calicivirus, and panleukopenia.
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DOI：
10.4142/jvs.2021.22.e38
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发表时间：
2021-05
影响因子：
1.8
通讯作者：
Thongkorn K
中科院分区：
文献类型：
--
作者：
Songaksorn N;Petsophonsakul W;Pringproa K;Lampang KN;Sthitmatee N;Srifawattana N;Piyarungsri K;Thongkorn K
猫病毒性鼻气管炎、杯状病毒和泛白细胞减少症（FVRCP）疫苗是由在克兰德尔-里斯猫肾细胞系中培养的病毒制备的，可以诱导抗体与猫肾组织发生交叉反应。本研究调查了猫肾组织自身抗体的患病率及其与FVRCP疫苗接种频率的关系。采集了156只活猫和26只死猫的血清和肾脏样本。采用酶联免疫吸附试验（ELISA）检测肾组织抗体和FVRCP抗原抗体，采用免疫荧光法检测肾结合抗体模式。抗体识别蛋白采用Western blot分析。ELISA和免疫荧光结果显示，猫肾组织自身抗体的检出率分别为41%和13%。在近端和远端小管的间质细胞、根尖边缘和细胞质中观察到肾结合抗体；抗体分别与分子量为40、47、38和20 kDa的蛋白结合。疫苗接种与抗肾抗体之间没有直接联系，但肾脏组织抗体阳性与抗fvrcp抗体显著相关。在有FVRCP抗体的猫中发现自身抗体的比值比或相关性是没有FVRCP抗体的猫的2.8倍。这些初步结果表明抗fvrcp与抗猫肾组织之间存在关联。然而，不能直接证明重复接种会增加诱导肾结合抗体的风险。扩大样本量和随访这些自身抗体是否会导致肾功能损害将是有趣的。
The feline viral rhinotracheitis, calicivirus, and panleukopenia (FVRCP) vaccine, prepared from viruses grown in the Crandell-Rees feline kidney cell line, can induce antibodies to cross-react with feline kidney tissues. This study surveyed the prevalence of autoantibodies to feline kidney tissues and their association with the frequency of FVRCP vaccination. Serum samples and kidneys were collected from 156 live and 26 cadaveric cats. Antibodies that bind to kidney tissues and antibodies to the FVRCP antigen were determined by enzyme-linked immunosorbent assay (ELISA), and kidney-bound antibody patterns were investigated by examining immunofluorescence. Proteins recognized by antibodies were identified by Western blot analysis. The prevalences of autoantibodies that bind to kidney tissues in cats were 41% and 13% by ELISA and immunofluorescence, respectively. Kidney-bound antibodies were observed at interstitial cells, apical border, and cytoplasm of proximal and distal tubules; the antibodies were bound to proteins with molecular weights of 40, 47, 38, and 20 kDa. There was no direct link between vaccination and anti-kidney antibodies, but positive antibodies to kidney tissues were significantly associated with the anti-FVRCP antibody. The odds ratio or association in finding the autoantibody in cats with the antibody to FVRCP was 2.8 times higher than that in cats without the antibody to FVRCP. These preliminary results demonstrate an association between anti-FVRCP and anti-cat kidney tissues. However, an increase in the risk of inducing kidney-bound antibodies by repeat vaccinations could not be shown directly. It will be interesting to expand the sample size and follow-up on whether these autoantibodies can lead to kidney function impairment.
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DOI：
10.2460/javma.2002.220.38
发表时间：
2002-01-01
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Lappin, MR;Andrews, J;Jensen, WA
通讯作者：
Jensen, WA
针对猫的疫苗接种：小猫的一项现场研究的影响。
影响因子：
2.6
作者：
Jakel V;Cussler K;Hanschmann KM;Truyen U;König M;Kamphuis E;Duchow K
通讯作者：
Duchow K
影响因子：
4.6
作者：
Kochagul, Varankpicha;Srivorakul, Saralee;Pringproa, Kidsadagon
通讯作者：
Pringproa, Kidsadagon
影响因子：
5.5
作者：
HogenEsch, H
通讯作者：
HogenEsch, H
影响因子：
1
作者：
Lappin, MR;Jensen, WA;Hawley, JR
通讯作者：
Hawley, JR