Insight into transketolase of Pyropia haitanensis under desiccation stress based on integrative analysis of omics and transformation
Insight into transketolase of Pyropia haitanensis under desiccation stress based on integrative analysis of omics and transformation
复制标题
基于组学与转化的综合分析揭示干旱胁迫下坛紫菜转酮醇酶
DOI：
10.1186/s12870-019-2076-4
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发表时间：
2019-11
影响因子：
5.3
通讯作者：
Xie chaotian
中科院分区：
文献类型：
--
作者：
Shi jianzhi;Wang wenlei;Lin yinghui;Xu kai;Xu yan;Ji dehua;Chen changsheng;Xie chaotian
海地焦蝇（pyropia haitanensis）分布于潮间带，可承受90%以上的失水。然而，启用p的机制。海地人在恶劣条件下生存的能力仍然是未知的。阐明p的机制。我们对haitanensis的耐干燥性进行了转录组和蛋白质组的综合分析，并对其进行了转基因的携带aP. haitanensis基因的reinhardchlamydomonas进行了分析。海棠迅速调整其生理活动以补偿高达60%的水分损失，此后，光合作用、抗氧化系统、伴侣蛋白和细胞骨架被激活以应对严重的干燥胁迫。综合分析表明，转酮醇酶（TKL）受到所有干燥处理的影响。TransgenicC。过度表达phtkl2的细胞在渗透胁迫下的生长优于野生型细胞。海田菊在转录组和蛋白质组方面迅速建立适应稳态，以确保其菌体在复水后能够恢复。此外，PhTKLis对p至关重要。haitanensisdesiccation宽容。目前的数据可能提供新的见解，为藻类和植物育种表现出增强的干燥耐受性。
BackgroundPyropia haitanensis, distributes in the intertidal zone, can tolerate water losses exceeding 90%. However, the mechanisms enablingP. haitanensisto survive harsh conditions remain uncharacterized. To elucidate the mechanism underlyingP. haitanensisdesiccation tolerance, we completed an integrated analysis of its transcriptome and proteome as well as transgenicChlamydomonas reinhardtiicarrying aP. haitanensisgene.ResultsP. haitanensisrapidly adjusted its physiological activities to compensate for water losses up to 60%, after which, photosynthesis, antioxidant systems, chaperones, and cytoskeleton were activated to response to severe desiccation stress. The integrative analysis suggested that transketolase (TKL) was affected by all desiccation treatments. TransgenicC. reinhardtiicells overexpressedPhTKLgrew better than the wild-type cells in response to osmotic stress.ConclusionP. haitanensisquickly establishes acclimatory homeostasis regarding its transcriptome and proteome to ensure its thalli can recover after being rehydrated. Additionally,PhTKLis vital forP. haitanensisdesiccation tolerance. The present data may provide new insights for the breeding of algae and plants exhibiting enhanced desiccation tolerance.
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影响因子：
4.6
作者：
Fan M;Sun X;Xu N;Liao Z;Li Y;Wang J;Fan Y;Cui D;Li P;Miao Z
通讯作者：
Miao Z
影响因子：
5.3
作者：
Pan L;Meng C;Wang J;Ma X;Fan X;Yang Z;Zhou M;Zhang X
通讯作者：
Zhang X
影响因子：
3
作者：
Yeonju Na;Ha-Nul Lee;Jiwoong Wi;W. Jeong;D. Choi
通讯作者：
Yeonju Na;Ha-Nul Lee;Jiwoong Wi;W. Jeong;D. Choi
影响因子：
4.3
作者：
Holzinger A;Herburger K;Kaplan F;Lewis LA
通讯作者：
Lewis LA
影响因子：
--
作者：
Stincone A;Prigione A;Cramer T;Wamelink MM;Campbell K;Cheung E;Olin-Sandoval V;Grüning NM;Krüger A;Tauqeer Alam M;Keller MA;Breitenbach M;Brindle KM;Rabinowitz JD;Ralser M
通讯作者：
Ralser M