Genomic investigation refutes record of most diverged avian hybrid.
Genomic investigation refutes record of most diverged avian hybrid.
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DOI：
10.1002/ece3.9689
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发表时间：
2023-01
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
--
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1957年报道了记录在案的最分歧的鸟类杂交种（Numida mangagris × Penelope superciliaris）。这一鉴定尚未得到证实，与大多数当代杂交研究一样，鉴定是基于表型的，这可能会产生误导。在这项研究中，我们测序的标本的问题，并进行分析，以验证标本的亲子关系。我们在一个专门的古代DNA设施中从标本中提取DNA，并进行全基因组短读测序。我们使用BLAST来找到与杂交样本读数相似的鸡形目序列。我们发现，BLAST命中的比例压倒性地映射到两个物种，N。鸭肉和原鸡。此外，我们使用鸟类直系同源物构建了同源基因，并解析了作为杂交后代姐妹的物种。再次，杂交标本被放置为N的姐妹。tagris和G.鸡。尽管不是N.虽然这种杂交种是由美洲斑和P. superciliaris杂交而成，但它仍然是遗传数据证实的最具分歧的鸟类杂交种。除了纠正最分歧的鸟类杂交的“记录”之外，这些发现支持最近的断言，即鸟类杂交的形态和行为鉴定可能容易出错。因此，这项研究为杂交研究者提供了一个警示。在这篇文章中，Alfieri及其同事重新鉴定了一种历史上的鸟类杂交种，并利用现代遗传技术纠正了这种鉴定，同时也纠正了最分歧的鸟类杂交种的记录。
The most diverged avian hybrid that has been documented (Numida meleagris × Penelope superciliaris) was reported in 1957. This identification has yet to be confirmed, and like most contemporary studies of hybridization, the identification was based on phenotype, which can be misleading. In this study, we sequenced the specimen in question and performed analyses to validate the specimen's parentage. We extracted DNA from the specimen in a dedicated ancient DNA facility and performed whole‐genome short‐read sequencing. We used BLAST to find Galliformes sequences similar to the hybrid specimen reads. We found that the proportion of BLAST hits mapped overwhelmingly to two species, N. meleagris and Gallus gallus. Additionally, we constructed phylogenies using avian orthologs and parsed the species placed as sister to the hybrid. Again, the hybrid specimen was placed as a sister to N. meleagris and G. gallus. Despite not being a hybrid between N. meleagris and P. superciliaris, the hybrid still represents the most diverged avian hybrid confirmed with genetic data. In addition to correcting the “record” of the most diverged avian hybrid, these findings support recent assertions that morphological and behavioral‐based identifications of avian hybrids can be error‐prone. Consequently, this study serves as a cautionary tale to researchers of hybridization. In this article, Alfieri and colleagues revisit the identification of a historic avian hybrid, and using modern genetic techniques, correct the identification, while also correcting the record of the most diverged avian hybrid.
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