DNA solution conformation via infrared circular dichroism: experimental and theoretical results for B-family polymers.
DNA solution conformation via infrared circular dichroism: experimental and theoretical results for B-family polymers.
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通过红外圆二色性观察 DNA 溶液构象：B 族聚合物的实验和理论结果。
DOI：
10.1021/bi00484a018
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Diem,M
中科院分区：
文献类型：
--
作者：
Zhong,WX;Gulotta,M;Goss,DJ;Diem,M
化学系，纽约城市大学，亨特学院，695 Park Avenue，纽约，纽约10021接收于1989年11月13日;修订版手稿接收于1990年3月28日摘要：红外已经观察到DNA模型d（CG）s、poly（dG-dC）-poly（dG-dC）、poly（dG）·poly（dC）、poly（dG）·poly（dC）、poly（dG）·poly（dC）的（振动）圆二色性（VCD）。聚（dA-dT）-聚（dA-dT）和聚（dA>聚（dT）在缓冲水溶液中的B-构象。用耦合羰基伸缩振动态的激子模型对观测结果进行了定量解释。（在红外光谱区域中观察到的振动跃迁中的圆二色性最近已被用于阐明核酸溶液构象（Annamalai和Keiderling，1987; Gulotta等，1989年）。对于核酸，这项研究得到了美国国家科学基金会（CHE 86-07070 to DJG）、美国心脏协会-纽约市设立的调查员（DJG）、美国国立卫生研究院（GM 28619 to MD）和几个纽约城市大学教师研究奖的支持。所用的VCD仪器是用以前NSF向MD（CHE 86-07934）提供的资金建造的。DNA结构的计算进行了部门的分子建模设施，根据RCMI赠款RR 03037购买。电子CD和VCD信号都是由位于碱基内的（电子或振动）跃迁的偶极耦合产生的。这些跃迁相互作用或耦合，产生吸收和圆二色性，这些特征由激子理论（Tinoco，1963）支配。然而，VCD提供了优于传统电子CD的优势，在可见/UV光谱区域观察到更多和更窄的光谱特征。此外，对于某些跃迁，例如羰基伸缩振动，产生光谱特征的振动模式被很好地定域化，并且偶极变化发生在几乎平行于连接碳和氧的键的方向上
Department of Chemistry, City University of New York, Hunter College, 695 Park Avenue, New York, New York 10021 Received November 13, 1989; Revised Manuscript Received March 28, 1990 abstract: Infrared (vibrational) circular dichroism (VCD) has been observed for the DNA models d (CG) s, poly (dG-dC)-poly (dG-dC), poly (dG)«poly (dC), poly (dA-dT)-poly (dA-dT), and poly (dA> poly (dT) in the B-conformation in buffered, aqueous solution. The observed results are quantitatively interpreted in terms of the exciton model for coupled carbonyl stretchingvibrational states.(circular dichroism in vibrational transitions, observed in the infrared spectral region, has recently been applied to the elucidation of nucleic acid solution conformation (Annamalai & Keiderling, 1987; Gulotta et al., 1989). For nucleic acids, tThis research was supported by the National Science Foundation (CHE 86-07070 to DJG), an American Heart Association-NYC Es-tablished Investigatorship (DJG), the National Institutes of Health (GM 28619 to MD), and several City University of New York Faculty Research Awards. The VCD instrument utilized was constructed with funds derived from a previous NSF grant to MD (CHE 86-07934). DNA structural computations were carried out on the departmental molecular modeling facility, purchased under RCMI Grant RR 03037. both electronicCD and VCD signals arise from the dipolar coupling of (electronic or vibrational) transitions localized within the bases. These transitions interact, or couple, and produce absorption and circular dichroismfeatures that are governed by the exciton theory (Tinoco, 1963). However, VCD offersthe advantage over conventional electronic CD, observed in the visible/UV spectral region, of more and narrower spectral features. In addition, for certain transitions, such as carbonyl stretching vibrations, the vibrational modes giving rise to the spectral features are well localized, and the dipole change occurs in a direction that is nearly parallel to the bond connecting the carbon and oxygen
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DOI：
10.1073/pnas.85.12.4392
发表时间：
1988-06-01
影响因子：
11.1
作者：
JACKSON, IJ
通讯作者：
JACKSON, IJ
使用胱胺对小鼠血红蛋白 (Hbb) 表型进行简化分型
影响因子：
2.4
作者：
J. B. WhitneyIII
通讯作者：
J. B. WhitneyIII
DOI：
10.1016/s0006-291x(88)81370-6
发表时间：
1988-06-30
影响因子：
3.1
作者：
KWON, BS;WAKULCHIK, M;KESTLER, D
通讯作者：
KESTLER, D
DOI：
10.1016/0006-291x(64)90359-6
发表时间：
1964-01-01
影响因子：
3.1
作者：
POMERANTZ, SH
通讯作者：
POMERANTZ, SH
影响因子：
9.8
作者：
B. de Martinville;A. Wyman;R. White;U. Francke
通讯作者：
B. de Martinville;A. Wyman;R. White;U. Francke