Aging features of the migratory locust at physiological and transcriptional levels.
Aging features of the migratory locust at physiological and transcriptional levels.
复制标题
飞蝗生理和转录水平的衰老特征
DOI：
10.1186/s12864-021-07585-3
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发表时间：
2021-04-10
期刊：
影响因子：
4.4
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Guo S;Yang P;Liang B;Zhou F;Hou L;Kang L;Wang X
背景非果蝇纲动物为衰老生物学研究提供了多样的衰老类型和重要的补充系统。然而，很少有人注意到非果蝇昆虫在衰老研究中的特殊作用。在这里，年龄相关的功能的飞蝗，飞蝗，确定在生理，细胞和转录水平。ResultsIn生理评估，飞行性能和精子状态的蝗虫显示明确的年龄相关的下降在男性成年人。转录分析表明蝗虫与模式物种具有相似的衰老相关基因。但与果蝇和哺乳动物不同，蝗虫器官特异性衰老转录特征表现为飞行肌和脂肪体表达变化强烈，而脑中转录变化较小。飞行肌和脂肪体衰老的主要转录特征分别是蛋白相关基因和解毒与吞噬基因表达的变化。细胞评估显示，线粒体异常的发生率显着增加，在老年飞行肌肉，细胞凋亡信号和核异常的增强，而不是在大脑中。此外，一些众所周知的衰老基因和蝗虫衰老相关基因（即，结论本研究揭示了蝗虫衰老的多层次特征，为进一步研究蝗虫衰老机制奠定了基础。
BackgroundNon-Drosophilainsects provide diverse aging types and important complementary systems for studies of aging biology. However, little attention has been paid to the special roles of non-Drosophilainsects in aging research. Here, the aging-related features of the migratory locust,Locusta migratoria, were determined at the physiological, cellular, and transcriptional levels.ResultsIn physiological assessments, the flight performance and sperm state of locusts displayed clear aging-related decline in male adults. Transcriptional analyses demonstrated locusts have similar aging-related genes with model species. However, different from those ofDrosophilaand mammals, the organ-specific aging transcriptional features of locusts were characterized by intensive expression changes in flight muscle and fat body and little transcriptional changes in brain. The predominant transcriptional characteristics of flight muscle and fat body aging were changes in expression of mitochondrion-related genes and detoxification and phagocytosis genes, respectively. Cellular assessments revealed the incidence of mitochondrial abnormalities significantly increased in aged flight muscle, and apoptotic signals and nuclear abnormalities were enhanced in aged fat body but not in brain. In addition, some well-known aging genes and locust aging-related genes (i.e.,IAP1,PGRP-SA, andLIPT1), whose roles in aging regulation were rarely reported, were demonstrated to affect lifespan, metabolism, and flight ability of locusts after RNAi.ConclusionThis study revealed multi-level aging signatures of locust, thus laying a foundation for further investigation of aging mechanisms in this famous insect in the future.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
7.8
作者：
Baumgart M;Groth M;Priebe S;Savino A;Testa G;Dix A;Ripa R;Spallotta F;Gaetano C;Ori M;Terzibasi Tozzini E;Guthke R;Platzer M;Cellerino A
通讯作者：
Cellerino A
影响因子：
64.5
作者：
Davie K;Janssens J;Koldere D;De Waegeneer M;Pech U;Kreft Ł;Aibar S;Makhzami S;Christiaens V;Bravo González-Blas C;Poovathingal S;Hulselmans G;Spanier KI;Moerman T;Vanspauwen B;Geurs S;Voet T;Lammertyn J;Thienpont B;Liu S;Konstantinides N;Fiers M;Verstreken P;Aerts S
通讯作者：
Aerts S
影响因子：
64.8
作者：
Herndon, LA;Schmeissner, PJ;Driscoll, M
通讯作者：
Driscoll, M
影响因子：
2.7
作者：
Boerjan, Bart;Sas, Filip;De Loof, Arnold
通讯作者：
De Loof, Arnold