Taxonomic assessment of two wild house mouse subspecies using whole-genome sequencing.
Taxonomic assessment of two wild house mouse subspecies using whole-genome sequencing.
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DOI：
10.1038/s41598-022-25420-x
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发表时间：
2022-12-02
影响因子：
4.6
通讯作者：
Dumont, Beth L.
中科院分区：
文献类型：
--
作者：
Lawal, Raman Akinyanju;Mathis, Verity L.;Barter, Mary E.;Charette, Jeremy R.;Garretson, Alexis;Dumont, Beth L.
家鼠物种复合体（Mus musculus）由三个主要亚种组成。基于有限数量的标记的不同形态和分子变异，还提出了大量次要亚种。虽然主要小家鼠亚种之间的系统发育关系已明确，但次要亚种之间以及次要亚种与主要亚种之间的关系仍不太清楚。在这里，我们整合了博物馆保存的来自一个次要亚种（M. m. bactrianus）的家鼠样本的从头基因组测序以及先前被定性为 M. m. bactrianus 的家鼠的公开基因组序列。 helgolandicus，具有来自三个主要家鼠亚种不同代表的全基因组序列。我们表明，分配给次要 M. m. 的小鼠。 bactrianus 和 M. m. helgolandicus 亚种与 M. m. 没有遗传差异。栗和 M. m.分别是家畜。总的来说，我们的工作表明 M. m. bactrianus 和 M. m. helgolandicus 亚种不是合理的分类实体，强调利用全基因组序列数据为亚种命名提供信息的重要性。此外，我们的研究提供了从风干小鼠皮肤生成全基因组序列的定制实验程序，以及关键基因组资源，为未来野生小鼠多样性的基因组研究提供信息。
The house mouse species complex (Mus musculus) is comprised of three primary subspecies. A large number of secondary subspecies have also been suggested on the basis of divergent morphology and molecular variation at limited numbers of markers. While the phylogenetic relationships among the primary M. musculus subspecies are well-defined, relationships among secondary subspecies and between secondary and primary subspecies remain less clear. Here, we integrate de novo genome sequencing of museum-stored specimens of house mice from one secondary subspecies (M. m. bactrianus) and publicly available genome sequences of house mice previously characterized as M. m. helgolandicus, with whole genome sequences from diverse representatives of the three primary house mouse subspecies. We show that mice assigned to the secondary M. m. bactrianus and M. m. helgolandicus subspecies are not genetically differentiated from M. m. castaneus and M. m. domesticus, respectively. Overall, our work suggests that the M. m. bactrianus and M. m. helgolandicus subspecies are not well-justified taxonomic entities, emphasizing the importance of leveraging whole-genome sequence data to inform subspecies designations. Additionally, our investigation provides tailored experimental procedures for generating whole genome sequences from air-dried mouse skins, along with key genomic resources to inform future genomic studies of wild mouse diversity.
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影响因子：
3.3
作者：
Fujiwara, Kazumichi;Kawai, Yosuke;Takada, Toyoyuki;Shiroishi, Toshihiko;Saitou, Naruya;Suzuki, Hitoshi;Osada, Naoki
通讯作者：
Osada, Naoki
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影响因子：
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通讯作者：
Starmer, Joshua
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9.2
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Chang CC;Chow CC;Tellier LC;Vattikuti S;Purcell SM;Lee JJ
通讯作者：
Lee JJ
影响因子：
9.8
作者：
Harr, Bettina;Karakoc, Emre;Neme, Rafik;Teschke, Meike;Pfeifle, Christine;Pezer, Zeljka;Babiker, Hiba;Linnenbrink, Miriam;Montero, Inka;Scavetta, Rick;Abai, Mohammad Reza;Puente Molins, Marta;Schlegel, Mathias;Ulrich, Rainer G.;Altmueller, Janine;Franitza, Marek;Buentge, Anna;Kuenzel, Sven;Tautz, Diethard
通讯作者：
Tautz, Diethard