Influenza virus-induced dendritic cell maturation is associated with the induction of strong T cell immunity to a coadministered, normally nonimmunogenic protein.
Influenza virus-induced dendritic cell maturation is associated with the induction of strong T cell immunity to a coadministered, normally nonimmunogenic protein.
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DOI：
10.1084/jem.20030266
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发表时间：
2003-07-07
期刊：
影响因子：
--
通讯作者：
Moran TM
中科院分区：
文献类型：
--
作者：
Brimnes MK;Bonifaz L;Steinman RM;Moran TM
我们评估的建议，在微生物感染，树突状细胞（DC）经历成熟，并提出了来自微生物的肽以及无害的环境抗原的混合物。在不存在或存在流感病毒的情况下，将小鼠暴露于不含内毒素的卵清蛋白（OVA）的气雾剂。在其不存在的情况下，OVA不能诱导B和T细胞应答，甚至耐受，但与流感，OVA特异性抗体和CD 8+细胞溶解性T淋巴细胞的发展。无论是否感染，OVA选择性地存在于引流纵隔淋巴结中，如通过输注的CD 8+和CD 4 + TCR转基因T细胞的相当增殖所评估的。在没有流感的情况下，这些OVA特异性T细胞产生很少的IL-2，IL-4，IL-10和IFN-γ，但在感染时，CD 4+和CD 8 + T细胞产生高水平的IL-2和IFN-γ。OVA加流感处理的小鼠也显示出对重组牛痘OVA病毒攻击的加速恢复。来自感染小鼠纵隔淋巴结的CD 11 c + DCs选择性刺激OVA和流感特异性T细胞并经历成熟，具有较高水平的MHC II类、CD 80和CD 86分子。相对缓慢（2-3天）的成熟动力学密切相关的时间，在此期间，OVA吸入引起特异性抗体。因此，呼吸道感染可以诱导DC成熟，同时诱导B和T细胞对同时吸入的无害抗原的免疫。
We evaluated the proposal that during microbial infection, dendritic cells (DCs) undergo maturation and present a mixture of peptides derived from the microbe as well as harmless environmental antigens. Mice were exposed to an aerosol of endotoxin free ovalbumin (OVA) in the absence or presence of influenza virus. In its absence, OVA failed to induce B and T cell responses and even tolerized, but with influenza, OVA-specific antibodies and CD8+ cytolytic T lymphocytes developed. With or without infection, OVA was presented selectively in the draining mediastinal lymph nodes, as assessed by the comparable proliferation of infused, CD8+ and CD4+, TCR transgenic T cells. In the absence of influenza, these OVA-specific T cells produced little IL-2, IL-4, IL-10, and IFN-γ, but with infection, both CD4+ and CD8+ T cells made high levels of IL-2 and IFN-γ. The OVA plus influenza-treated mice also showed accelerated recovery to a challenge with recombinant vaccinia OVA virus. CD11c+ DCs from the mediastinal lymph nodes of infected mice selectively stimulated both OVA- and influenza-specific T cells and underwent maturation, with higher levels of MHC class II, CD80, and CD86 molecules. The relatively slow (2–3 d) kinetics of maturation correlated closely to the time at which OVA inhalation elicited specific antibodies. Therefore respiratory infection can induce DC maturation and simultaneously B and T cell immunity to an innocuous antigen inhaled concurrently.
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DOI：
10.1159/000235107
发表时间：
1990-02-01
期刊：
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY
影响因子：
--
作者：
HOLT, PG;SCHONHEGRAD, MA;MCMENAMIN, PG
通讯作者：
MCMENAMIN, PG
影响因子：
15.3
作者：
Bonifaz, L;Bonnyay, D;Mahnke, K;Rivera, M;Nussenzweig, MC;Steinman, RM
通讯作者：
Steinman, RM
DOI：
10.1084/jem.194.6.769
发表时间：
2001-09-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hawiger D;Inaba K;Dorsett Y;Guo M;Mahnke K;Rivera M;Ravetch JV;Steinman RM;Nussenzweig MC
通讯作者：
Nussenzweig MC
DOI：
10.1159/000234615
发表时间：
1988-05-01
期刊：
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY
影响因子：
--
作者：
LEIBOVITZ, E;FREIHORST, J;OGRA, PL
通讯作者：
OGRA, PL
影响因子：
15.3
作者：
Crowley, M;Inaba, K;Steinman, R M
通讯作者：
Steinman, R M