Lactobacillus gasseri SBT2055 reduces infection by and colonization of Campylobacter jejuni.
Lactobacillus gasseri SBT2055 reduces infection by and colonization of Campylobacter jejuni.
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Gasseri SBT2055乳酸杆菌通过弯曲杆菌空肠的感染和定殖。
DOI：
10.1371/journal.pone.0108827
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Mukai T
中科院分区：
文献类型：
--
作者：
Nishiyama K;Seto Y;Yoshioka K;Kakuda T;Takai S;Yamamoto Y;Mukai T
弯曲杆菌是鸡肠道的正常居民。人体内这种微生物的病原性感染伴随着肠粘膜表面的严重炎症。本研究旨在评价加氏乳杆菌SBT 2055（LG 2055）体外抑制空肠弯曲菌粘附和侵袭以及抑制C.雏鸡体内空肠定殖。用LG 2055预处理显著降低了C.空肠81-176。甲醇（MeOH）固定的LG 2055也减少了C.空肠81-176。然而，蛋白酶K（ProK）处理的LG 2055消除抑制作用。此外，LG 2055与C.空肠81-176。ProK处理阻止了这种共聚集，表明由LG 2055的蛋白质细胞表面组分介导的共聚集表型对于减少C.空肠81-176粘连和浸润。在体内测定中，在用C.空肠81-176。在接种后14天，用LG 2055处理的雏鸡显著减少了盲肠中的梭菌定殖。空肠。减少C的数量。jejuni 81-176细胞粘附并被人上皮细胞内化，证明LG 2055是一种有效和竞争性排除C.空肠81-176。此外，鸡定殖测定的结果表明，用LG 2055处理可用于抑制C.空肠定植的雏鸡在早期生长阶段。
Campylobacter is a normal inhabitant of the chicken gut. Pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. The aim of this study was to evaluate the ability of Lactobacillus gasseri SBT2055 (LG2055) to inhibit the adhesion and invasion of Campylobacter jejuni in vitro and to suppress C. jejuni colonization of chicks in vivo. Pretreatment with LG2055 significantly reduced adhesion to and invasion of a human epithelial cell line, Intestine 407, by C. jejuni 81–176. Methanol (MeOH)-fixed LG2055 also reduced infection by C. jejuni 81–176. However, proteinase K (ProK)-treated LG2055 eliminated the inhibitory effects. Moreover, LG2055 co-aggregated with C. jejuni 81–176. ProK treatment prevented this co-aggregation, indicating that the co-aggregation phenotype mediated by the proteinaceous cell-surface components of LG2055 is important for reducing C. jejuni 81–176 adhesion and invasion. In an in vivo assay, oral doses of LG2055 were administered to chicks daily for 14 days after oral inoculation with C. jejuni 81–176. At 14 days post-inoculation, chicks treated with LG2055 had significantly reduced cecum colonization by C. jejuni. Reduction in the number of C. jejuni 81–176 cells adhering to and internalized by human epithelial cells demonstrated that LG2055 is an organism that effectively and competitively excludes C. jejuni 81–176. In addition, the results of the chick colonization assay suggest that treatment with LG2055 could be useful in suppressing C. jejuni colonization of the chicks at early growth stages.
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影响因子：
1.6
作者：
Kawai, Y;Saitoh, B;Itoh, T
通讯作者：
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影响因子：
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作者：
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