CRP Involved in Nile Tilapia (Oreochromis niloticus) against Bacterial Infection.
CRP Involved in Nile Tilapia (Oreochromis niloticus) against Bacterial Infection.
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作者：
C反应蛋白（CRP）是一种急性时相蛋白，可用作炎症的早期诊断标志物。从硬骨鱼中分离到的CRP很少，鱼类CRP的特异性免疫功能及其作用机制尚未得到充分研究。因此，本研究从尼罗罗非鱼中鉴定了CRP基因，并研究了其在细菌感染过程中的作用。目前的研究结果表明，CRP通过凝集细菌、调节吞噬和炎症反应参与抗细菌免疫反应。本研究为进一步了解鱼类CRP对细菌感染的保护机制提供了依据。C-反应蛋白（CRP）是一种急性时相蛋白，可作为炎症的早期诊断标志物，也是一种进化上保守的蛋白质，从节肢动物到哺乳动物都有发现。然而，CRP在尼罗罗非鱼（Oreochromis niloticus）免疫应答中的作用尚不清楚。在这项研究中，从尼罗罗非鱼CRP基因（On-CRP）的鉴定，并在体内或体外细菌感染的反应中的作用进行了研究。On-CRP含有一个675 bp的开放阅读框，编码一个224个氨基酸的多肽，具有保守的正五聚蛋白结构域。On-CRP与其他鱼类物种的同一性超过50%，与哺乳动物的同一性超过30%。On-CRP在肝脏中的转录水平最高，感染无乳链球菌和嗜水气单胞菌后，On-CRP的转录水平显著升高。体外实验结果表明，On-CRP重组蛋白能提高单核/巨噬细胞的吞噬活性，并具有钙依赖性的细菌凝集活性。体内外实验均表明On-CRP可促进炎症反应并激活补体途径。然而，CRP和几种免疫途径之间的直接关系尚不能得到证实。本研究为进一步探讨CRP保护鱼类免受细菌感染的机制奠定了理论基础。
C-reactive protein (CRP) is an acute-phase protein that can be used as an early diagnostic marker for inflammation. Few CRPs have been isolated from teleost, and the specific immunological functions and mechanism of fish CRP have not been well-studied. Therefore, in this research, a CRP gene from Nile tilapia was identified, and its roles during bacterial infection were investigated. The current results revealed that CRP participated in anti-bacterial immune response through agglutinating bacterial, regulating phagocytosis and inflammation. Hopefully, our data might be beneficial in further study to understand the protective mechanism of fish CRP against bacterial infection. C-reactive protein (CRP) is an acute-phase protein that can be used as an early diagnostic marker for inflammation, which is also an evolutionarily conserved protein and has been identified from arthropods to mammals. However, the roles of CRP during the immune response of Nile tilapia (Oreochromis niloticus) remain unclear. In this study, a CRP gene from Nile tilapia (On-CRP) was identified, and its roles in response to bacterial infection were investigated in vivo or in vitro. On-CRP was found to contain an open reading frame of 675 bp, encoding a polypeptide of 224 amino acids with the conservative pentraxin domain. On-CRP shares more than 50% of its identity with other fish species, and 30% of its identity with mammals. The transcriptional level of On-CRP was most abundant in the liver and its transcripts can be remarkably induced following Streptococcus agalactiae and Aeromonas hydrophila infection. Furthermore, in vitro analysis indicated that the recombinant protein of On-CRP improved phagocytic activity of monocytes/macrophages, and possessed a bacterial agglutination activity in a calcium-dependent manner. Both in vivo and in vitro experiments indicated that On-CRP could promote inflammation and activate the complement pathway. However, a direct relationship between CRP and several immune pathways could not be confirmed. The present data lays a theoretical foundation to further explore the mechanism of how CRP protects fish against bacterial infection.
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C反应蛋白/血清淀粉样蛋白P促进弹涂鱼、大弹涂鱼的促炎功能并诱导单核细胞/巨噬细胞的M1型极化
影响因子：
4.7
作者：
Cai，Shi-Yu;Nie，Li;Chen，Jiong
通讯作者：
Chen，Jiong
影响因子：
4.5
作者：
Li, Qi;Liu, Ruonan;Hou, Xitan
通讯作者：
Hou, Xitan
影响因子：
4.7
作者：
Li, Mo-fei;Chen, Cheng;Sun, Li
通讯作者：
Sun, Li
影响因子：
4.4
作者：
Conte MA;Gammerdinger WJ;Bartie KL;Penman DJ;Kocher TD
通讯作者：
Kocher TD
DOI：
10.1084/jem.123.2.365
发表时间：
1966-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hurlimann J;Thorbecke GJ;Hochwald GM
通讯作者：
Hochwald GM