Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures.
Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structures.
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DOI：
10.1038/nprot.2009.176
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发表时间：
2009
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
--
作者：
The anisotropic nanofluidic filter (nanofilter) array (ANA) is a unique molecular sieving structure for separating biomolecules. Here we describe fabrication of planar and vertical ANA chips and how to perform continuous-flow bioseparation using them. This protocol is most useful for bioengineers that are interested in developing automated multistep chip-based bioanalysis systems and assumes prior cleanroom microfabrication knowledge. The ANA consists of a two-dimensional periodic nanofilter array, and the designed structural anisotropy of the ANA causes different sized- or charged-biomolecules to follow distinct trajectories under applied electric fields, leading to efficient continuous-flow separation. Using microfluidic channels surrounding the ANA, the fractionated biomolecule streams are collected and routed to different fluid channels or reservoirs for convenient sample recovery and downstream bioanalysis. The ANA is physically robust and can be reused repeatedly. Compared to conventional gel-based separation techniques, the ANA offers the potential for faster separation, higher throughput, and more convenient sample recovery.
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影响因子：
2.9
作者：
Bow, Hansen;Fu, Jianping;Han, Jongyoon
通讯作者：
Han, Jongyoon
影响因子：
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Mao, P;Han, JY
通讯作者：
Han, JY
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56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC
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38.3
作者：
Fu, Jianping;Schoch, Reto B.;Han, Jongyoon
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Han, Jongyoon
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Cao, H;Yu, ZN;Chou, SY
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Chou, SY