A distinct type of cell in myocardium: interstitial Cajal-like cells (ICLCs).
A distinct type of cell in myocardium: interstitial Cajal-like cells (ICLCs).
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DOI：
10.1111/j.1582-4934.2008.00668.x
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发表时间：
2009-02
影响因子：
5.3
通讯作者：
Popescu LM
中科院分区：
文献类型：
--
作者：
Kostin S;Popescu LM
2005年首次报道了心脏中存在一种新型的间质细胞，即间质Cajal样细胞（ICLC）。它们的鉴定主要基于超微结构标准：非常长（数十至数百微米）和念珠状突起，非常薄（小于0.2 μm），低于光学显微镜的分辨率。还通过亚甲蓝活体染色、银浸渍和CD 34、波形蛋白、CD 117/c-kit等免疫反应性鉴定了心肌ICLCs。尽管一系列研究提供了人心房和大鼠心室中存在ICLCs的证据，但需要在其他实验室使用其他技术进行进一步调查，以证实这些结果的一致性。在这里，我们提供了进一步的证据ICLC在人类和哺乳动物心脏的存在（通过透射和扫描电子显微镜，以及共聚焦激光扫描显微镜）。值得注意的是，我们证实ICLC通过脱落（微）囊泡与相邻细胞进行通信。虽然这些所谓的ICLC代表了一种独特的细胞类型，不同于经典的Cajal间质细胞或成纤维细胞，但它们在心肌中的作用仍有待确定。提出了几个假设：（i）成体基质（间充质）干细胞，其可能参与心脏修复/重塑;（ii）细胞间信号传导（例如通过脱落微泡）;（iii）化学机械转换器和（iv）起搏和/或心律失常发生中的参与者，等等。
The existence of a novel type of interstitial cells in the heart, interstitial Cajal-like cells (ICLCs), had been described for the first time in 2005. Their identification was mainly based on ultrastructural criteria: very long (tens up to hundreds of micrometres) and moniliform prolongations, which are extremely thin (less than 0.2 μm), below the resolving power of light microscopy. Myocardial ICLCs were also identified by methylene-blue vital staining, silver impregnation, and immunoreactivity for CD 34, vimentin, CD117/c-kit, etc. Although a series of studies provided evidence for the existence of ICLCs in human atria and rat ventricles, further investigations in other laboratories, using additional techniques, are required to substantiate the consistency of these findings. Here we provide further evidence for the existence of ICLCs in human and mammalian hearts (by transmission and scanning electron microscopy, as well as confocal laser scanning microscopy). Noteworthy, we confirm that ICLCs communicate with neighbouring cells via shedding (micro)vesicles. Although these so-called ICLCs represent a distinct type of cells, different from classical interstitial cells of Cajal, or fibroblasts, their role(s) in myocardium remain(s) to be established. Several hypotheses are proposed: (i) adult stromal (mesenchymal) stem cells, which might participate in cardiac repair/remodelling; (ii) intercellular signalling (e.g. via shedding microvesicles); (iii) chemo-mechanical transducers and (iv) players in pacemaking and/or arrhytmogenesis, and so on.
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影响因子：
5.3
作者：
Eyden B
通讯作者：
Eyden B
DOI：
10.1111/j.1582-4934.2006.tb00306.x
发表时间：
2006-01
影响因子：
5.3
作者：
Hinescu ME;Gherghiceanu M;Mandache E;Ciontea SM;Popescu LM
通讯作者：
Popescu LM
影响因子：
3.6
作者：
Bobryshev, YV
通讯作者：
Bobryshev, YV
影响因子：
4.7
作者：
Cong Xin Huang;Cheng Lin Hu;Yan Bo Li
通讯作者：
Yan Bo Li
影响因子：
3.2
作者：
Hinescu, Mihail E.;Ardeleanu, Carmen;Popescu, Laurentziu M.
通讯作者：
Popescu, Laurentziu M.