A multicenter evaluation of diagnostic tools to define endpoints for programs to eliminate bancroftian filariasis.
A multicenter evaluation of diagnostic tools to define endpoints for programs to eliminate bancroftian filariasis.
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DOI：
10.1371/journal.pntd.0001479
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发表时间：
2012-01
影响因子：
3.8
通讯作者：
Lammie P
中科院分区：
文献类型：
--
作者：
Gass K;Beau de Rochars MV;Boakye D;Bradley M;Fischer PU;Gyapong J;Itoh M;Ituaso-Conway N;Joseph H;Kyelem D;Laney SJ;Legrand AM;Liyanage TS;Melrose W;Mohammed K;Pilotte N;Ottesen EA;Plichart C;Ramaiah K;Rao RU;Talbot J;Weil GJ;Williams SA;Won KY;Lammie P
成功的大规模药物管理（MDA）运动使几个国家接近消灭淋巴丝虫病（LF）。需要一种诊断工具来确定流行水平何时下降到可以停止MDA运动而不会有复发的威胁的程度。进行了一项六国研究，评估了七种诊断检测方法的性能，包括微丝蚴检测（血涂片、PCR）、寄生虫抗原检测（ICT、Og4C3）和抗丝虫抗体检测（Bm14、PanLF、尿液SXP）。在每个国家进行了一次社区调查和一次学校调查。共有来自六个国家的8 513人参加了这项研究，其中6 443人通过社区调查，2 070人通过学校调查。这些参与者的样本被用于进行49，585次诊断测试。每个测试都被认为有积极和消极的属性，但总体而言，ICT测试被发现是76%的敏感性检测微丝蚴血症和93%的特异性，在确定个人阴性的微丝蚴和抗丝虫抗体; Og4C3测试是87%的敏感性和95%的特异性。然而，我们的结论是，信息和通信技术应该是主要的工具，建议有关停止MDA的决策。作为一种即时诊断，信通技术相对便宜，不需要实验室设备，具有令人满意的灵敏度和特异性，并可在10分钟内处理-质量符合方案使用要求。Og4C3提供了一种令人满意的基于实验室的诊断替代方案。淋巴丝虫病（LF）是一种蚊媒寄生虫病，是消除的候选者，主要是因为大规模药物管理（MDA）运动的成功，其中整个高危人群每年一次接受两种药物的单剂量治疗方案。因此，需要一种诊断工具来确定LF的流行率何时低于持续传播的阈值，以便可以停止MDA计划。为了确定可用的最佳诊断工具，进行了一项多国研究，以评估七种诊断检测对一组患者标本的性能。选择最有效的诊断测试是基于对每种测试的准确性、技术要求、方案可行性和可靠性以及对测试性能的信心的评价。这项研究发现了每种测试的优点和缺点。根据数据和经验，确定ICT检测（一种床旁快速卡检测）是用于定义MDA终点的首选诊断工具，尽管Og4C3检测提供了一种合适的基于实验室的替代方法。
Successful mass drug administration (MDA) campaigns have brought several countries near the point of Lymphatic Filariasis (LF) elimination. A diagnostic tool is needed to determine when the prevalence levels have decreased to a point that MDA campaigns can be discontinued without the threat of recrudescence. A six-country study was conducted assessing the performance of seven diagnostic tests, including tests for microfilariae (blood smear, PCR), parasite antigen (ICT, Og4C3) and antifilarial antibody (Bm14, PanLF, Urine SXP). One community survey and one school survey were performed in each country. A total of 8,513 people from the six countries participated in the study, 6,443 through community surveys and 2,070 through school surveys. Specimens from these participants were used to conduct 49,585 diagnostic tests. Each test was seen to have both positive and negative attributes, but overall, the ICT test was found to be 76% sensitive at detecting microfilaremia and 93% specific at identifying individuals negative for both microfilariae and antifilarial antibody; the Og4C3 test was 87% sensitive and 95% specific. We conclude, however, that the ICT should be the primary tool recommended for decision-making about stopping MDAs. As a point-of-care diagnostic, the ICT is relatively inexpensive, requires no laboratory equipment, has satisfactory sensitivity and specificity and can be processed in 10 minutes—qualities consistent with programmatic use. Og4C3 provides a satisfactory laboratory-based diagnostic alternative. Lymphatic filariasis (LF), a mosquito-borne parasitic disease, is a candidate for elimination largely because of the success of mass drug administration (MDA) campaigns, in which entire at-risk populations are given a once-yearly regimen of single-dose treatment with two medications. As a result, a diagnostic tool is needed to determine when the prevalence of LF has fallen below the threshold for sustained transmission so that MDA programs can be stopped. To determine the best diagnostic tool available, a multi-country study was conducted to assess the performance of seven diagnostic tests on a panel of patient specimens. The selection of the most effective diagnostic test was based on an evaluation of each test's accuracy, technical requirements, programmatic feasibility and reliability, as well as confidence in test performance. This study found advantages and disadvantages to each test. Based on the data and experiences it was determined that the ICT test, a point-of-care rapid card test, is the preferred diagnostic tool for use in defining the end-point of MDA, although the Og4C3 test provides a suitable laboratory-based alternative.
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影响因子：
3.8
作者：
Ottesen, Eric A.;Hooper, Pamela J.;Biswas, Gautam
通讯作者：
Biswas, Gautam
DOI：
10.4269/ajtmh.2001.65.362
发表时间：
2001-10-01
影响因子：
3.3
作者：
Itoh, M;Weerasooriya, MV;Kimura, E
通讯作者：
Kimura, E
影响因子：
168.9
作者：
Ramzy, RMR;El Setouhy, M;Weil, GJ
通讯作者：
Weil, GJ
DOI：
10.1590/s0074-02762009000400015
发表时间：
2009-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Rocha, Abraham;Braga, Cynthia;Furtado, André
通讯作者：
Furtado, André
影响因子：
6.4
作者：
CHANTEAU, S;MOULIAPELAT, JP;CARTEL, JL
通讯作者：
CARTEL, JL