Silver nanowire exposure results in internalization and toxicity to Daphnia magna.
Silver nanowire exposure results in internalization and toxicity to Daphnia magna.
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DOI：
10.1021/nn4034103
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发表时间：
2013-12-23
期刊：
影响因子：
17.1
通讯作者：
Gilbert, Benjamin
中科院分区：
文献类型：
--
作者：
Scanlan, Leona D.;Reed, Robert B.;Loguinov, Alexandre V.;Antczak, Philipp;Tagmount, Abderrahmane;Aloni, Shaul;Nowinski, Daniel Thomas;Luong, Pauline;Tran, Christine;Karunaratne, Nadeeka;Don Pham;Lin, Xin Xin;Falciani, Francesco;Higgins, Christopher P.;Ranville, James F.;Vulpe, Chris D.;Gilbert, Benjamin
关键词：
纳米线（NW），高纵横比的纳米材料，越来越多地用于技术材料和消费品，并可能具有不同于纳米颗粒的毒理学特性。我们进行了全面的评价的物理化学稳定性的两种尺寸和涂层的四个银纳米线（AgNWs）和它们的毒性大型蚤。无机铝掺杂的二氧化硅涂层是有效的，比有机聚（乙烯基吡咯烷酮）涂层在防止银氧化或Ag+释放，并经历了一个显着的形态转化后，在一个小时内添加到低离子强度的水蚤生长介质。所有AgNW对D.大但比离子银毒性小。毒性不同的AgNW尺寸，涂层和溶液化学的功能。媒体中的Ag+释放不能解释所观察到的AgNW毒性。单粒子电感耦合等离子体质谱（spICPMS）区分和定量溶解和纳米颗粒银在微升规模体积的大型蚤血淋巴的检测限约为10 ppb。接触Ag+和AgNW的水蚤血淋巴内的银水平达到或超过了生长培养基中的初始浓度，表明在过滤器喂养过程中有效积累。银纳米线和Ag+暴露后，血淋巴中银的主要形式是富银颗粒。干燥血淋巴的扫描电子显微镜（SEM）成像发现AgNW和银沉淀物均不存在于AgNW原液或生长培养基中。AgNW上的有机和无机涂层都在摄入或吸收过程中发生了变化。基因表达响应的途径、基因本体和聚类分析表明AgNWs对大型蚤的影响不同于离子型银。
Nanowires (NWs), high-aspect-ratio nanomaterials, are increasingly used in technological materials and consumer products and may have toxicological characteristics distinct from nanoparticles. We carried out a comprehensive evaluation of the physico-chemical stability of four silver nanowires (AgNWs) of two sizes and coatings and their toxicity to Daphnia magna. Inorganic aluminum-doped silica coatings were less effective than organic poly(vinyl pyrrolidone) coatings at preventing silver oxidation or Ag+ release and underwent a significant morphological transformation within one-hour following addition to low ionic strength Daphnia growth media. All AgNWs were highly toxic to D. magna but less toxic than ionic silver. Toxicity varied as a function of AgNW dimension, coating and solution chemistry. Ag+ release in the media could not account for observed AgNW toxicity. Single-particle inductively coupled plasma mass spectrometry (spICPMS) distinguished and quantified dissolved and nanoparticulate silver in microliter-scale volumes of Daphnia magna hemolymph with a limit of detection of approximately 10 ppb. The silver levels within the hemolymph of Daphnia exposed to both Ag+ and AgNW met or exceeded the initial concentration in the growth medium, indicating effective accumulation during filter feeding. Silver-rich particles were the predominant form of silver in hemolymph following exposure to both AgNWs and Ag+. Scanning electron microscopy (SEM) imaging of dried hemolymph found both AgNWs and silver precipitates that were not present in the AgNW stock or the growth medium. Both organic and inorganic coatings on the AgNW were transformed during ingestion or absorption. Pathway, gene ontology and clustering analyses of gene expression response indicated effects of AgNWs distinct from ionic silver on Daphnia magna.
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影响因子：
17.1
作者：
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