Susceptibility of rats of different ages to inoculation with swine haemagglutinating encephalomyelitis virus (a coronavirus) by various routes.
Susceptibility of rats of different ages to inoculation with swine haemagglutinating encephalomyelitis virus (a coronavirus) by various routes.
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DOI：
10.1053/jcpa.2001.0471
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发表时间：
2001-07
影响因子：
0.8
通讯作者：
Tohyama K
中科院分区：
文献类型：
--
作者：
Hirano N;Haga S;Sada Y;Tohyama K
用血凝性脑脊髓炎病毒67 N株通过脑内（i.c.）鼻内（i.n.），腹膜内（i. p.），皮下（s.c.），静脉内（i. v.）和口服给药剂量分级。感染途径按疗效降序排列为：i.ci.ns.ci.pi.v.和口服。1周龄和2周龄的大鼠在死亡率和平均死亡时间方面基本相似，无论接种途径如何，但经口途径影响不大。与1周龄和2周龄大鼠相比，4周龄大鼠对所有途径的病毒均较不敏感。八周龄的大鼠通过i.c.n.或s.c.所有通过其他途径接种的人都活了下来。为了跟踪病毒在中枢神经系统中的传播，将通过i.c.路线进行了检查。病毒首先在第1天在大脑中检测到，第2天在脊髓中检测到。两种组织中的病毒滴度在第4天达到107空斑形成单位（PFU）/0.2 g的平台，此时已出现临床体征。免疫组织化学分析发现，病毒特异性抗原首先出现在海马和大脑皮质的锥体细胞中，随后出现在脑桥和脊髓的大型神经元中。再晚些时候（第4天）免疫标记被发现在小脑浦肯野细胞，但不是在室管膜细胞，脉络丛或其他胶质细胞。
Haemagglutinating encephalomyelitis virus, strain 67N, was used to inoculate 1-, 2-, 4- and 8-week-old rats by the intracerebral (i.c.), intranasal (i.n.), intraperitoneal (i.p.), subcutaneous (s.c.), intravenous (i.v.) and oral routes with graded doses. The routes of infection, in descending order of efficacy, were: i.ci.ns.ci.pi.v. and oral. Rats aged 1 and 2 weeks were generally similar in terms of mortality and mean time to death, regardless of inoculation route, except for the oral route, which had little effect. In comparison with the 1- and 2-week-old rats, the 4-week-old rats were less susceptible to the virus by all routes. Eight-week-old rats inoculated by the i.ci.n. or s.c. routes died, but all those inoculated by other routes survived. To follow the spread of virus in the central nervous system, 4-week-old rats inoculated by the i.c. route were examined. The virus was first detected in the brain on day 1 and in the spinal cord on day 2. The viral titres in both tissues reached a plateau of 107plaque-forming units (PFU)/0·2 g by day 4, at which time clinical signs had developed. By immunohistochemical analysis, virus-specific antigen was found first in the pyramidal cells of the hippocampus and cerebral cortex, and later in the large-sized neurons of the pons and spinal cord. Still later (day 4) immunolabelling was found in Purkinje cells of the cerebellum, but not in the ependymal cells, choroid plexus or other glial cells.
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影响因子：
0.8
作者：
NARITA, M;KAWAMURA, H;KOBAYASHI, M
通讯作者：
KOBAYASHI, M
影响因子：
0.8
作者：
YAGAMI, K;HIRAI, K;HIRANO, N
通讯作者：
HIRANO, N
影响因子：
6.4
作者：
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通讯作者：
HARRISON, AK
影响因子：
2.2
作者：
KERSHAW, GF;WILSON, AA
通讯作者：
WILSON, AA
影响因子：
3.1
作者：
Hirano N;Ono K;Takasawa H;Murakami T;Haga S
通讯作者：
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