Replication and plaque formation of swine hemagglutinating encephalomyelitis virus (67N) in swine cell line, SK-K culture.
Replication and plaque formation of swine hemagglutinating encephalomyelitis virus (67N) in swine cell line, SK-K culture.
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DOI：
10.1016/0166-0934(90)90149-a
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发表时间：
1990-01
影响因子：
3.1
通讯作者：
Haga S
中科院分区：
文献类型：
--
作者：
Hirano N;Ono K;Takasawa H;Murakami T;Haga S
猪血凝性脑脊髓炎病毒（HEV）67 N株在猪SK-K细胞中生长迅速，并产生细胞病变（CPE），即融合细胞和圆形细胞从玻璃表面脱落。在SK-K细胞单层中用未稀释的培养液进一步传代后，CPE发展较早，并在接种后48小时内（p.i.）完成。用兔抗鼠传病毒血清间接免疫荧光法在感染的SK-K细胞胞浆中检测到病毒特异性抗原。SK-K传代病毒以及原始小鼠传代病毒在简单覆盖培养基下在SK-K细胞单层上形成清晰的噬斑。通过对影响HEV 67 N空斑形成的各种因素的研究，建立了HEV 67 N空斑检测系统。通过该系统，在感染后48小时，在感染培养物的流体相中检测到超过106 PFU/0.2ml的病毒产量。SK-K-传代病毒通过脑内接种引起4周龄小鼠的致死性感染，但被兔抗小鼠传代病毒的抗血清抑制。噬斑形成和血凝活性的病毒特异性抑制抗血清对小鼠传代和SK-K传代67 N病毒。
Swine hemagglutinating encephalomyelitis virus (HEV), 67N strain, adapted to suckling mouse brain, grew readily in a porcine cell line, SK-K cell culture with cytopathic effect (CPE) consisting of syncytium formation and detachment of fused cells and round cells from glass surface. After further passages in SK-K cell monolayers with undiluted culture fluid, CPE developed earlier and became complete within 48 h postinoculation (p.i.). Viral specific antigen was detected in the cytoplasm of the infected SK-K cells by indirect immunofluorescence using rabbit antiserum against the mouse-passaged virus. The SK-K-passaged virus as well as the original mouse-passaged virus formed clear plaques on SK-K cell monolayers under simple overlay medium. The plaque assay system for HEV 67N was established by studying various factors influencing the plaque formation in the SK-K cell cultures. By this system more than 106 PFU/0.2 ml of the virus yield was detected in the fluid phase of the infected cultures at 48 h p.i. The SK-K-passaged virus caused fatal infection in 4-week-old mice by intracerebral inoculation, but was inhibited by rabbit antiserum against the mouse-passaged virus. Plaque formation and hemagglutinating activity of the virus were specifically inhibited by antisera against the mouse-passaged and SK-K-passaged 67N virus.
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影响因子：
3.3
作者：
YAMAGISHI, H;NAGAMINE, T;MATUMOTO, M
通讯作者：
MATUMOTO, M
影响因子：
0.8
作者：
YAGAMI, K;HIRAI, K;HIRANO, N
通讯作者：
HIRANO, N
影响因子：
6.4
作者：
KAYE, HS;YARBROUGH, WB;HARRISON, AK
通讯作者：
HARRISON, AK
影响因子：
4.6
作者：
SIDDELL, SG;ANDERSON, R;VANDERZEIJST, BAM
通讯作者：
VANDERZEIJST, BAM
影响因子：
3.3
作者：
SATO, K;INABA, Y;MATUMOTO, M
通讯作者：
MATUMOTO, M