Expansion of Signal Transduction Pathways in Fungi by Extensive Genome Duplication.
Expansion of Signal Transduction Pathways in Fungi by Extensive Genome Duplication.
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DOI：
10.1016/j.cub.2016.04.038
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发表时间：
2016-06-20
期刊：
影响因子：
9.2
通讯作者：
Grigoriev, Igor V.
中科院分区：
文献类型：
--
作者：
Corrochano, Luis M.;Kuo, Alan;Marcet-Houben, Marina;Polaino, Silvia;Salamov, Asaf;Villalobos-Escobedo, Jose M.;Grimwood, Jane;Isabel Alvarez, M.;Avalos, Javier;Bauer, Diane;Benito, Ernesto P.;Benoit, Isabelle;Burger, Gertraud;Camino, Lola P.;Canovas, David;Cerda-Olmedo, Enrique;Cheng, Jan-Fang;Dominguez, Angel;Elias, Marek;Eslava, Arturo P.;Glaser, Fabian;Gutierrez, Gabriel;Heitman, Joseph;Henrissat, Bernard;Iturriaga, Enrique A.;Lang, B. Franz;Lavin, Jose L.;Lee, Soo Chan;Li, Wenjun;Lindquist, Erika;Lopez-Garcia, Sergio;Luque, Eva M.;Marcos, Ana T.;Martin, Joel;McCluskey, Kevin;Medina, Humberto R.;Miralles-Duran, Alejandro;Miyazaki, Atsushi;Munoz-Torres, Elisa;Oguiza, Jose A.;Ohm, Robin A.;Olmedo, Maria;Orejas, Margarita;Ortiz-Castellanos, Lucila;Pisabarro, Antonio G.;Rodriguez-Romero, Julio;Ruiz-Herrera, Jose;Ruiz-Vazquez, Rosa;Sanz, Catalina;Schackwitz, Wendy;Shahriari, Mahdi;Shelest, Ekaterina;Silva-Franco, Fatima;Soanes, Darren;Syed, Khajamohiddin;Tagua, Victor G.;Talbot, Nicholas J.;Thon, Michael R.;Tice, Hope;de Vries, Ronald P.;Wiebenga, Ad;Yadav, Jagjit S.;Braun, Edward L.;Baker, Scott E.;Garre, Victoriano;Schmutz, Jeremy;Horwitz, Benjamin A.;Torres-Martinez, Santiago;Idnurm, Alexander;Herrera-Estrella, Alfredo;Gabaldon, Toni;Grigoriev, Igor V.
植物和真菌利用光和其他信号来调节发育、生长和新陈代谢。真菌黑藻菌的子实体是对环境信号（包括光）做出反应的单细胞，但其机制在很大程度上是未知的。相关真菌环毛霉是一种机会性的人类病原体，它在接收到来自环境的信号后改变其生长模式以促进发病。了解这些生物如何对环境信号作出反应，有助于深入了解单个真核细胞的感觉知觉和信号转导机制，以及它们在发病机制中的作用。我们对P. blakesleeanus和M. circinelloides的基因组进行了测序，并表明它们是由广泛的基因组重复或最有可能的全基因组重复（WGD）形成的，这在真菌中很少观察到。我们表明，基因组复制扩大了基因家族，包括那些参与信号转导的基因，并且复制的基因已经特化，正如它们在光调节方面的差异所证明的那样。对光的转录反应随发育阶段的不同而变化，在一个光感受器突变体中仍然观察到。一个具有光感受器基因madA杂合突变的嗜光突变体表明，光感受器剂量对信号转导的大小很重要。我们得出的结论是，基因组复制提供了改善信号转导的手段，增强了对环境信号的感知。我们的研究结果将有助于理解基因组动力学在真核生物感官知觉进化中的作用。
Plants and fungi use light and other signals to regulate development, growth, and metabolism. The fruiting bodies of the fungus Phycomyces blakesleeanus are single cells that react to environmental cues, including light, but the mechanisms are largely unknown. The related fungus Mucor circinelloides is an opportunistic human pathogen that changes its mode of growth upon receipt of signals from the environment to facilitate pathogenesis. Understanding how these organisms respond to environmental cues should provide insights into the mechanisms of sensory perception and signal transduction by a single eukaryotic cell, and their role in pathogenesis. We sequenced the genomes of P. blakesleeanus and M. circinelloides, and show that they have been shaped by an extensive genome duplication or, most likely, a whole genome duplication (WGD), which is rarely observed in fungi. We show that the genome duplication has expanded gene families, including those involved in signal transduction, and that duplicated genes have specialized, as evidenced by differences in their regulation by light. The transcriptional response to light varies with the developmental stage and is still observed in a photoreceptor mutant of P. blakesleeanus. A phototropic mutant of P. blakesleeanus with a heterozygous mutation in the photoreceptor gene madA demonstrates that photosensor dosage is important for the magnitude of signal transduction. We conclude that the genome duplication provided the means to improve signal transduction for enhanced perception of environmental signals. Our results will help to understand the role of genome dynamics in the evolution of sensory perception in eukaryotes.
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影响因子：
3.4
作者：
Marcet-Houben M;Marceddu G;Gabaldón T
通讯作者：
Gabaldón T
DOI：
10.1007/bf00315807
发表时间：
1990-08-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
CAMPUZANO, V;DIAZMINGUEZ, JM;ALVAREZ, MI
通讯作者：
ALVAREZ, MI
影响因子：
14.9
作者：
Nicolas FE;Moxon S;de Haro JP;Calo S;Grigoriev IV;Torres-Martínez S;Moulton V;Ruiz-Vázquez RM;Dalmay T
通讯作者：
Dalmay T
DOI：
10.1073/pnas.0600633103
发表时间：
2006-03-21
影响因子：
11.1
作者：
Idnurm, A;Rodríguez-Romero, J;Heitman, J
通讯作者：
Heitman, J
影响因子：
3.7
作者：
Chaudhary, Suman;Polaino, Silvia;Idnurm, Alexander
通讯作者：
Idnurm, Alexander