A balanced diet is necessary for proper entrainment signals of the mouse liver clock.
A balanced diet is necessary for proper entrainment signals of the mouse liver clock.
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对于适当的小鼠肝钟的夹带信号是必需的。
DOI：
10.1371/journal.pone.0006909
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发表时间：
2009-09-07
期刊：
影响因子：
3.7
通讯作者：
Shibata S
中科院分区：
文献类型：
--
作者：
Hirao A;Tahara Y;Kimura I;Shibata S
小鼠的外周生物钟不仅受到光暗周期的影响，而且还受到每日限制喂养时间表的影响。行为和细胞培养实验表明，葡萄糖水平的增加作为一个因素，在这种喂养诱导的夹带。对于应用于人类的喂养诱导夹带，营养成分和饮食的变化应予以考虑。为了阐明饮食夹带所需的食物组成，我们研究了是否完全或部分替代饮食营养素影响小鼠肝脏时钟的相移。与禁食小鼠或随意喂养的小鼠相比，在给予标准小鼠饮食，即AIN-93 M配方[0.6-0.85 g/10 g小鼠BW]（组成：14%酪蛋白、47%玉米淀粉、15%糊化玉米淀粉、10%糖、4%大豆油和10%其他[纤维、维生素、矿物质等]）的Per 2：：荧光素酶敲入小鼠中，肝脏生物发光节律在中间一天提前3-4 h，了2天当每种营养素单独测试（100%的营养素），一个微不足道的弱相位提前被发现诱导玉米淀粉和大豆油，但几乎没有相位提前诱导糊化玉米淀粉，高直链淀粉玉米淀粉，葡萄糖，蔗糖，或酪蛋白。没有油、维生素或纤维的葡萄糖和酪蛋白的组合引起显著的相位提前。当AIN-93 M中的玉米淀粉被葡萄糖、蔗糖、果糖、聚葡萄糖、高直链玉米淀粉或糊化玉米淀粉取代时，相位提前的幅度超过血糖浓度的增加。我们的研究结果强烈表明：（1）含有碳水化合物/糖和蛋白质的均衡饮食有利于限制喂养诱导的外周昼夜节律钟的夹带，（2）增加血糖的均衡饮食，但不仅仅是糖，适合夹带。这些发现可能有助于为航空旅行者和轮班工人提供机上膳食的饮食建议，以减少时差症状。
The peripheral circadian clock in mice is entrained not only by light-dark cycles but also by daily restricted feeding schedules. Behavioral and cell culture experiments suggest an increase in glucose level as a factor in such feeding-induced entrainment. For application of feeding-induced entrainment in humans, nutrient content and dietary variations should be considered. To elucidate the food composition necessary for dietary entrainment, we examined whether complete or partial substitution of dietary nutrients affected phase shifts in liver clocks of mice. Compared with fasting mice or ad libitum fed mice, the liver bioluminescence rhythm advanced by 3–4 h on the middle day in Per2::luciferase knock-in mice that were administered a standard mouse diet, i.e. AIN-93M formula [0.6–0.85 g/10 g mouse BW] (composition: 14% casein, 47% cornstarch, 15% gelatinized cornstarch, 10% sugar, 4% soybean oil, and 10% other [fiber, vitamins, minerals, etc.]), for 2 days. When each nutrient was tested alone (100% nutrient), an insignificant weak phase advance was found to be induced by cornstarch and soybean oil, but almost no phase advance was induced by gelatinized cornstarch, high-amylose cornstarch, glucose, sucrose, or casein. A combination of glucose and casein without oil, vitamin, or fiber caused a significant phase advance. When cornstarch in AIN-93M was substituted with glucose, sucrose, fructose, polydextrose, high-amylose cornstarch, or gelatinized cornstarch, the amplitude of phase advance paralleled the increase in blood glucose concentration. Our results strongly suggest the following: (1) balanced diets containing carbohydrates/sugars and proteins are good for restricted feeding-induced entrainment of the peripheral circadian clock and (2) a balanced diet that increases blood glucose, but not by sugar alone, is suitable for entrainment. These findings may assist in the development of dietary recommendations for on-board meals served to air travelers and shift workers to reduce jet lag-like symptoms.
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