Low Concentrations of Silver Nanoparticles Inhibit Spore Germination and Disturb Gender Differentiation of Ceratopteris thalictroides (L.) Brongn.
Low Concentrations of Silver Nanoparticles Inhibit Spore Germination and Disturb Gender Differentiation of Ceratopteris thalictroides (L.) Brongn.
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低浓度银纳米颗粒抑制孢子萌发并扰乱水蕨性别分化
DOI：
10.3390/nano12101730
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发表时间：
2022-05-18
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影响因子：
--
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中科院分区：
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银纳米粒子（AgNPs）因其优异的抗菌性能而被广泛应用于各行各业，这也导致了其被排放到水生环境中可能对水生植物产生负面影响。在本研究中，我们使用的水生蕨类植物，水蕨，作为一个模型，调查银纳米粒子对其孢子萌发，配子体，性别分化和生长的影响。结果表明，AgNPs显著抑制了C.在AgNP浓度高于0.02 mg/L时，此外，我们还发现银纳米颗粒对黄瓜配子体发育和生长的影响具有性别依赖性。海栖藻AgNP处理显著降低了配子体中两性体的比例和配子体的面积，而对雄配子体的影响不显著。使用AgNP滤液（无纳米颗粒）和AgNP加半胱氨酸（Ag+螯合剂），我们发现从纳米颗粒释放Ag+不是AgNP对C的毒性的原因。海栖藻AgNPs对孢子萌发的EC50为0.0492 mg/L，因此表明AgNPs对该物种的生态风险，即使浓度低于世卫组织饮用水质量指南的Ag元素浓度。
Because of their excellent antibacterial properties, silver nanoparticles (AgNPs) are widely used in all walks of life, which has caused them to be discharged into aquatic environments with possible negative effects on aquatic plants. In the present study, we used an aquatic fern, Ceratopteris thalictroides, as a model to investigate the effects of AgNPs on its spore germination, gametophytes, sex differentiation, and growth. The results demonstrated that AgNPs significantly inhibited spore germination of C. thalictroides at a AgNP concentration higher than 0.02 mg/L. Additionally, we found sex-dependent effects of AgNPs on the development and growth of the gametophyte of C. thalictroides. The proportion of hermaphrodites in the gametophytes and the area of gametophytes significantly decreased under AgNP treatment, while no significant effect was observed in the male gametophytes. Using the AgNP filtrate (without nanoparticles) and AgNPs plus cysteine (Ag+ chelator), we found that the release of Ag+ from nanoparticles was not the cause of the toxicity of AgNPs on C. thalictroides. The EC50 of AgNPs on spore germination was 0.0492 mg/L, thus indicating an ecological risk of AgNPs on this species even at concentrations lower than the Ag element concentration of the WHO guidelines for drinking-water quality.
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影响因子：
17.1
作者：
Bouwmeester, Hans;Poortman, Jenneke;Hendriksen, Peter J. M.
通讯作者：
Hendriksen, Peter J. M.
影响因子：
10.9
作者：
Gao, Jiejun;Lin, Lu;Sepulveda, Maria S.
通讯作者：
Sepulveda, Maria S.
影响因子：
5
作者：
Austin, Carlye A.;Umbreit, Thomas H.;Goering, Peter L.
通讯作者：
Goering, Peter L.
影响因子：
11.4
作者：
Geranio, L.;Heuberger, M.;Nowack, B.
通讯作者：
Nowack, B.
影响因子：
13.3
作者：
Jiang, Hong Sheng;Zhang, Yizhi;Li, Wei
通讯作者：
Li, Wei