Preliminary insights into the impact of primary radiochemotherapy on the salivary microbiome in head and neck squamous cell carcinoma.
Preliminary insights into the impact of primary radiochemotherapy on the salivary microbiome in head and neck squamous cell carcinoma.
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DOI：
10.1038/s41598-020-73515-0
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发表时间：
2020-10-06
影响因子：
4.6
通讯作者：
Wolf A
中科院分区：
文献类型：
--
作者：
Kumpitsch C;Moissl-Eichinger C;Pock J;Thurnher D;Wolf A
鳞状细胞癌是最常见的喉癌类型。治疗选择包括手术、放射治疗和/或化学（免疫）治疗。唾液微生物组是由疾病决定的，也可能是由治疗决定的，导致放化疗产生副作用，严重损害患者的健康。 16S rRNA 基因的高通量扩增子测序提供了研究健康和疾病中唾液微生物组变化的机会。在这项初步研究中，我们调查了健康受试者和头颈鳞状细胞癌患者在放化疗之前和接近终点（“之后”）唾液微生物组的细菌、真菌和古菌成分的变化。我们招募了 31 名患者和 11 名健康对照者，其中 11 名患者在放化疗前后提供了样本。分析显示对细菌和真菌微生物组有影响，具有部分拮抗反应，但对古菌微生物群落没有影响。具体来说，我们观察到放化疗后念珠菌特征个体增加，而治疗后微生物和真菌特征的总体多样性显着下降。因此，我们的研究表明，患者微生物组对放化疗有单独的反应，但具有未来优化疾病诊断和个性化治疗的潜力。
Squamous cell carcinoma is the most common type of throat cancer. Treatment options comprise surgery, radiotherapy, and/or chemo(immuno)therapy. The salivary microbiome is shaped by the disease, and likely by the treatment, resulting in side effects caused by chemoradiation that severely impair patients’ well-being. High-throughput amplicon sequencing of the 16S rRNA gene provides an opportunity to investigate changes in the salivary microbiome in health and disease. In this preliminary study, we investigated alterations in the bacterial, fungal, and archaeal components of the salivary microbiome between healthy subjects and patients with head and neck squamous cell carcinoma before and close to the end point of chemoradiation (“after”). We enrolled 31 patients and 11 healthy controls, with 11 patients providing samples both before and after chemoradiation. Analysis revealed an effect on the bacterial and fungal microbiome, with a partial antagonistic reaction but no effects on the archaeal microbial community. Specifically, we observed an individual increase in Candida signatures following chemoradiation, whereas the overall diversity of the microbial and fungal signatures decreased significantly after therapy. Thus, our study indicates that the patient microbiome reacts individually to chemoradiation but has potential for future optimization of disease diagnostics and personalized treatments.
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影响因子：
16.6
作者：
Arthur, Janelle C.;Gharaibeh, Raad Z.;Muehlbauer, Marcus;Perez-Chanona, Ernesto;Uronis, Joshua M.;McCafferty, Jonathan;Fodor, Anthony A.;Jobin, Christian
通讯作者：
Jobin, Christian
影响因子：
5.6
作者：
Banna GL;Torino F;Marletta F;Santagati M;Salemi R;Cannarozzo E;Falzone L;Ferraù F;Libra M
通讯作者：
Libra M
影响因子：
3.7
作者：
Schmidt BL;Kuczynski J;Bhattacharya A;Huey B;Corby PM;Queiroz EL;Nightingale K;Kerr AR;DeLacure MD;Veeramachaneni R;Olshen AB;Albertson DG
通讯作者：
Albertson DG
影响因子：
3.8
作者：
Almstahl, A.;Wikstrom, M.;Fagerberg-Mohlin, B.
通讯作者：
Fagerberg-Mohlin, B.
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J