Functional and morphological assessment of early impairment of airway function in a rat model of emphysema.
Functional and morphological assessment of early impairment of airway function in a rat model of emphysema.
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肺气肿大鼠模型气道功能早期损伤的功能和形态学评估。
DOI：
10.1152/japplphysiol.00587.2011
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hantos,Z
中科院分区：
文献类型：
--
作者：
Tolnai,J;Szabari,MV;Albu,G;Maar,BA;Parameswaran,H;Bartolak-Suki,E;Suki,B;Hantos,Z
本研究的目的是评估弹性蛋白酶诱导的肺气肿大鼠气道结构与功能的关系。用50 IU猪胰弹性蛋白酶（PPE，n= 8）或盐水（对照，n= 6）对Sprague-Dawley大鼠进行血管内治疗。6周后，测量肺容量[功能残气量（FRC）、残气量（RV）和总肺容量（TLC）]和低频阻抗参数（牛顿阻力，RN;组织阻尼;组织弹性，H），并在体内排气后缓慢充气至TLC期间记录气管音。将肺固定并染色用于标准形态测量、弹性蛋白和胶原蛋白。PPE组的FRC和RV高于对照组[分别为4.53 ± 0.7（SD）vs. 3.28 ± 0.45 ml;P= 0.003和1.06 ± 0.35 vs. 0.69 ± 0.18 ml;P= 0.036]，PPE治疗组大鼠的H小于对照组（1，344 ± 216 vs. 2，178 ± 305 cmH 2 O/l;P< 0.001），而RN无差异。两组每次充盈时的平均爆裂声数量相似;然而，爆裂声大小分布不同，PPE组的压力-容积曲线的下膝更高。显微镜图像显示两组肺泡大小分布不同，但支气管直径相似。治疗导致肺泡附着数量轻微但显著减少，弹性蛋白无差异，支气管壁中胶原蛋白的平均水平和异质性略有增加。这些结果表明，组织破坏不影响常规评估的气道阻力，在这个肺气肿模型，而在募集动力学的改变可以是气道功能受损的早期表现。
The aim of this study was to evaluate airway structure-function relations in elastase-induced emphysema in rats. Sprague-Dawley rats were treated intratracheally with 50 IU porcine pancreatic elastase (PPE,n= 8) or saline (controls,n= 6). Six weeks later, lung volumes [functional residual capacity (FRC), residual volume (RV), and total lung capacity (TLC)] and low-frequency impedance parameters (Newtonian resistance, RN; tissue damping; tissue elastance, H) were measured, and tracheal sounds were recorded during slow inflation to TLC following in vivo degassing. The lungs were fixed and stained for standard morphometry, elastin, and collagen. In the PPE group, FRC and RV were higher [4.53 ± 0.7 (SD) vs. 3.28 ± 0.45 ml;P= 0.003 and 1.06 ± 0.35 vs. 0.69 ± 0.18 ml;P= 0.036, respectively], and H was smaller in the PPE-treated rats than in the controls (1,344 ± 216 vs. 2,178 ± 305 cmH2O/l;P< 0.001), whereas there was no difference in RN. The average number of crackles per inflation was similar in the two groups; however, the crackle size distributions were different and the lower knee of the pressure-volume curves was higher in the PPE group. Microscopic images revealed different alveolar size distributions but similar bronchial diameters in the two groups. The treatment caused a slight but significant decrease in the numbers of alveolar attachments, no difference in elastin and slightly increased mean level and heterogeneity of collagen in the bronchial walls. These results suggest that tissue destruction did not affect the conventionally assessed airway resistance in this emphysema model, whereas the alterations in the recruitment dynamics can be an early manifestation of impaired airway function.
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慢性阻塞性肺疾病的病理生理学。
DOI：
10.1007/978-88-470-5647-3_22
发表时间：
1998
期刊：
Respiratory care clinics of North America
影响因子：
--
作者：
B. Celli
通讯作者：
B. Celli
预防性应用时，造血生长因子不会减少弹性蛋白酶诱导的大鼠肺气肿
影响因子：
3.5
作者：
A. Schmiedl;Tobias Lempa;H. Hoymann;S. Rittinghausen;D. Popa;T. Tschernig;H. Fehrenbach;R. Pabst;M. Hoeper;J. Hohlfeld
通讯作者：
J. Hohlfeld
气管内用弹性蛋白酶治疗的大鼠肺气肿模型。
影响因子：
8.3
作者：
E. Yokoyama;Z. Nambu;I. Uchiyama;H. Kyono
通讯作者：
H. Kyono
弹性蛋白酶诱导的肺气肿对大鼠气道对乙酰甲胆碱反应性的影响。
影响因子：
3.3
作者：
S. Bellofiore;D. Eidelman;P. Macklem;J. Martin
通讯作者：
J. Martin
在弹性蛋白酶诱导的大鼠肺气肿中，肺迷走神经反射和呼吸模式没有改变。
影响因子：
1.7
作者：
Mansoor,JK;Hyde,DM;Schelegie,ES
通讯作者：
Schelegie,ES