Strain-specific transfer of antibiotic resistance from an environmental plasmid to foodborne pathogens.
Strain-specific transfer of antibiotic resistance from an environmental plasmid to foodborne pathogens.
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DOI：
10.1155/2012/834598
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发表时间：
2012
影响因子：
--
通讯作者：
Boon N
中科院分区：
文献类型：
--
作者：
Van Meervenne E;Van Coillie E;Kerckhof FM;Devlieghere F;Herman L;De Gelder LS;Top EM;Boon N
对多种抗生素产生抗药性的病原体正在迅速出现，给人类健康带来重要后果。本研究探讨了从污水处理厂分离到的含有阿莫西林、链霉素、磺胺和四环素耐药基因的广宿主多重耐药质粒pB10是否可以转移到食源性致病菌沙门氏菌中。或大肠杆菌O157：H7，以及这种转移是如何改变受体的表型的。用平板法和流式细胞仪测定转染率。用纸片扩散法测定受体和转接子的耐药谱。在15个受体菌株中，有14个菌株检测到接合子。流式细胞仪检测转染率在6.8×10−9~3.0×10−2之间，转染率在1.0×10−5~1.9×10−2之间。除少数例外，转接子表现出明显的抗性增强，表明大部分抗性基因都得到了表达。综上所述，我们证明了环境质粒可以以高转移率转移到食源性致病菌中。然而，转移率似乎与受体菌株有关。此外，新获得的耐药基因可能会将抗生素敏感菌株转变为耐药菌株，为危害人类健康铺平道路。
Pathogens resistant to multiple antibiotics are rapidly emerging, entailing important consequences for human health. This study investigated if the broad-host-range multiresistance plasmid pB10, isolated from a wastewater treatment plant, harbouring amoxicillin, streptomycin, sulfonamide, and tetracycline resistance genes, was transferable to the foodborne pathogens Salmonella spp. or E. coli O157:H7 and how this transfer alters the phenotype of the recipients. The transfer ratio was determined by both plating and flow cytometry. Antibiotic resistance profiles were determined for both recipients and transconjugants using the disk diffusion method. For 14 of the 15 recipient strains, transconjugants were detected. Based on plating, transfer ratios were between 6.8 × 10−9 and 3.0 × 10−2 while using flow cytometry, transfer ratios were between <1.0 × 10−5 and 1.9 × 10−2. With a few exceptions, the transconjugants showed phenotypically increased resistance, indicating that most of the transferred resistance genes were expressed. In summary, we showed that an environmental plasmid can be transferred into foodborne pathogenic bacteria at high transfer ratios. However, the transfer ratio seemed to be recipient strain dependent. Moreover, the newly acquired resistance genes could turn antibiotic susceptible strains into resistant ones, paving the way to compromise human health.
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影响因子：
2.6
作者：
Bahl, Martin Iain;Burmolle, Mette;Sorensen, Soren J.
通讯作者：
Sorensen, Soren J.
影响因子：
4.4
作者：
Musovic, Sanin;Oregaard, Gunnar;Sorensen, Soren J.
通讯作者：
Sorensen, Soren J.
影响因子：
4.2
作者：
Boon, N;Depuydt, S;Verstraete, W
通讯作者：
Verstraete, W
影响因子：
3
作者：
Pourshaban, M;Ferrini, AM;Aureli, P
通讯作者：
Aureli, P
影响因子：
3.7
作者：
Fajardo A;Martínez-Martín N;Mercadillo M;Galán JC;Ghysels B;Matthijs S;Cornelis P;Wiehlmann L;Tümmler B;Baquero F;Martínez JL
通讯作者：
Martínez JL