Characterization of FA1654: A putative DPS protein in Filifactor alocis.
Characterization of FA1654: A putative DPS protein in Filifactor alocis.
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DOI：
10.1111/omi.12398
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发表时间：
2023-02
影响因子：
3.7
通讯作者：
Fletcher, Hansel M.
中科院分区：
文献类型：
--
作者：
Mangar, Malissa;Mishra, Arunima;Yang, Zhengrong;Deivanayagam, Champion;Fletcher, Hansel M.
Fillifactor alocis（一种挑剔的革兰氏阳性解糖厌氧菌）对牙周袋炎症环境的生存/适应需要克服氧化应激的能力。此外，其致病特征还体现在其在牙周袋氧化应激微环境中生存的能力以及可能调节微生物群落动态的能力。我们对其抗氧化应激机制及其对微生物生物膜毒力和致病性的影响的理解仍存在显着差距。上皮细胞与 F.alocis 和 P.gingivalis 的共感染导致包括 HMPREF0389_01654(FA1654) 在内的多个基因上调。生物信息学分析表明，FA1654 具有“双铁结合域”，可以充当 DNA 饥饿和固定相保护 (DPS) 蛋白。我们进一步表征了 FA1654 蛋白，以确定其在 F.alocis 抗氧化应激中的作用。在过氧化氢诱导的氧化应激存在下，F.alocis 中 FA1654 基因上调约 1.3 倍。在过氧化氢和铁存在的情况下，将纯化的 FA1654 蛋白与 DNA 一起孵育，可以保护 DNA 免受 Fenton 介导的降解。圆二色性 (CD) 和差示扫描荧光法 (DSF) 研究已证明 rFA1654 蛋白结合铁的内在能力，但 rFA1654 蛋白缺乏还原过氧化氢的内在能力。总的来说，这些数据可能表明 F.alocis 中的 FA1654 通过防止芬顿介导的氧化应激诱导的损伤的能力来参与氧化应激抵抗。
The survival/adaptation of Fillifactor alocis, a fastidious gram positive asaccharolytic anaerobe, to the inflammatory environment of the periodontal pocket requires an ability to overcome oxidative stress. Moreover, its pathogenic characteristics are highlighted by its capacity to survive in the oxidative-stress microenvironment of the periodontal pocket and a likely ability to modulate the microbial community dynamics. There is still a significant gap in our understanding of its mechanism of oxidative stress resistance and its impact on the virulence and pathogenicity of the microbial biofilm. Coinfection of epithelial cells with F.alocis and P.gingivalis, resulted in the upregulation of several genes including HMPREF0389_01654(FA1654). Bioinformatics analysis indicates that FA1654 has a “di-iron binding domain”, and could function as a DNA Starvation and Stationary Phase Protection (DPS) protein. We have further characterized the FA1654 protein to determine its role in oxidative stress resistance in F.alocis. In the presence of hydrogen peroxide induced oxidative stress there was a ~1.3 fold upregulation of the FA1654 gene in F.alocis. Incubation of the purified FA1654 protein with DNA in the presence of hydrogen peroxide and iron resulted in the protection of the DNA from Fenton-mediated degradation. Circular Dichroism (CD) and Differential Scanning Flourimetry (DSF) studies have documented the intrinsic ability of rFA1654 protein to bind iron, however the rFA1654 protein is missing the intrinsic ability to reduce hydrogen peroxide. Collectively, the data may suggest that FA1654 in F.alocis is involved in oxidative stress resistance via an ability to protect against Fenton-mediated oxidative stress-induced damage.
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影响因子：
3.1
作者：
Henry LG;McKenzie RM;Robles A;Fletcher HM
通讯作者：
Fletcher HM
影响因子：
5
作者：
de Alcantara, Nayra Rodrigues;de Oliveira, Fabio Muniz;Kipnis, Andre
通讯作者：
Kipnis, Andre
影响因子：
3.2
作者：
Armstrong, Cortney L.;Klaes, Christopher K.;Uriarte, Silvia M.
通讯作者：
Uriarte, Silvia M.
以 Filifactor alocis 为中心的共现组与不同口腔栖息地的牙周炎相关
影响因子：
4.6
作者：
Chen H;Liu Y;Zhang M;Wang G;Qi Z;Bridgewater L;Zhao L;Tang Z;Pang X
通讯作者：
Pang X
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ