A Filifactor alocis-centered co-occurrence group associates with periodontitis across different oral habitats.
A Filifactor alocis-centered co-occurrence group associates with periodontitis across different oral habitats.
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以 Filifactor alocis 为中心的共现组与不同口腔栖息地的牙周炎相关
DOI：
10.1038/srep09053
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发表时间：
2015-03-12
影响因子：
4.6
通讯作者：
Pang X
中科院分区：
文献类型：
--
作者：
Chen H;Liu Y;Zhang M;Wang G;Qi Z;Bridgewater L;Zhao L;Tang Z;Pang X
牙周炎是一种高度流行的多种微生物疾病，但涉及的多种口腔病原体的协同模式仍然很差的特点。在这里，收集了牙周炎患者和牙周健康志愿者的唾液、龈上和龈下菌斑样本，并采用16S rRNA基因焦磷酸测序进行分析。不同的口腔栖息地具有显着不同的微生物群，牙周炎和健康之间的微生物群组成的分离也被观察到。两步冗余分析确定了21个OTU，包括牙龈卟啉单胞菌，Tannerella alothia和Filifactor alocis，作为潜在的病原体，与牙周炎和两个牙周炎诊断参数（牙周袋深度和附着丧失）在唾液和龈上菌斑栖息地显着相关。有趣的是，21个OTU之间的成对相关分析显示，Filifactoralocis与其他7种推定的病原体正相关（R > 0.6，P < 0.05），形成了一个共现组，在牙周炎患者的所有三个生境中显著富集。这一细菌簇对牙周炎的诊断价值高于任何单个潜在病原体，尤其是唾液中的病原体。因此，我们的研究确定了一个潜在的协同生态模式，涉及8种共同感染的病原体在不同的口腔栖息地，为了解牙周炎的病因学和开发新的诊断和治疗提供了一个新的框架。
Periodontitis is a highly prevalent polymicrobial disease worldwide, yet the synergistic pattern of the multiple oral pathogens involved is still poorly characterized. Here, saliva, supragingival and subgingival plaque samples from periodontitis patients and periodontally healthy volunteers were collected and profiled with 16S rRNA gene pyrosequencing. Different oral habitats harbored significantly different microbiota, and segregation of microbiota composition between periodontitis and health was observed as well. Two-step redundancy analysis identified twenty-one OTUs, including Porphyromonas gingivalis, Tannerella forsythia and Filifactor alocis, as potential pathogens that were significantly associated with periodontitis and with two periodontitis diagnostic parameters (pocket depth and attachment loss) in both saliva and supragingival plaque habitats. Interestingly, pairwise correlation analysis among the 21 OTUs revealed that Filifactor alocis was positively correlated with seven other putative pathogens (R > 0.6, P < 0.05), forming a co-occurrence group that was remarkably enriched in all three habitats of periodontitis patients. This bacterial cluster showed a higher diagnostic value for periodontitis than did any individual potential pathogens, especially in saliva. Thus, our study identified a potential synergistic ecological pattern involving eight co-infecting pathogens across various oral habitats, providing a new framework for understanding the etiology of periodontitis and developing new diagnoses and therapies.
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影响因子：
11
作者：
Duran-Pinedo, Ana E.;Chen, Tsute;Frias-Lopez, Jorge
通讯作者：
Frias-Lopez, Jorge
影响因子：
3.7
作者：
Ge X;Rodriguez R;Trinh M;Gunsolley J;Xu P
通讯作者：
Xu P
影响因子：
--
作者：
Dahlén, G;Leonhardt, Å
通讯作者：
Leonhardt, Å
DOI：
10.1517/17530059.2011.542144
发表时间：
2011-01-01
期刊：
Expert opinion on medical diagnostics
影响因子：
--
作者：
Brinkmann, Ole;Zhang, Lei;Wong, David T
通讯作者：
Wong, David T
影响因子：
9.4
作者：
Kumar, Purnima S.;Leys, Eugene J.;Griffen, Ann L.
通讯作者：
Griffen, Ann L.