Discovery of the gray phenotype and white-gray-opaque tristable phenotypic transitions in Candida dubliniensis
Discovery of the gray phenotype and white-gray-opaque tristable phenotypic transitions in Candida dubliniensis
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都柏林念珠菌灰色表型和白灰不透明三稳态表型转变的发现
DOI：
10.1080/21505594.2015.1135287
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发表时间：
2016-02
期刊：
影响因子：
5.2
通讯作者：
Huang Guanghua
中科院分区：
文献类型：
--
作者：
Yue Huizhen;Hu Jian;Guan Guobo;Tao Li;Du Han;Li Houmin;Huang Guanghua
摘要 都柏林念珠菌与白色念珠菌密切相关，白色念珠菌是念珠菌病的主要病原体，主要与人类免疫缺陷病毒 (HIV) 阳性患者的口腔定植和感染有关。尽管这两个物种之间的基因组和表型特征高度相似，但都柏林念珠菌的毒性和流行程度均低于白色念珠菌。改变形态表型的能力是念珠菌属的一个显着特征，并且与毒力有关。在这项研究中，我们报告了 C. dubliniensis 的一种新表型，即灰色表型。与之前报道的白色和不透明细胞类型一起，灰色表型在都柏林念珠菌中形成三稳态表型转换系统，类似于白色念珠菌中的白-灰色-不透明三稳态转换系统。都柏林念珠菌的灰细胞在细胞形态、交配能力和遗传调控机制等几个生物学方面与白色念珠菌的灰细胞相似。然而，这两个物种的灰色表型有一些显着特征。例如，白色念珠菌灰细胞中的牛血清白蛋白 (BSA) 会诱导分泌型天冬氨酰蛋白酶 (Sap) 活性，但都柏林念珠菌的灰细胞中不会诱导分泌型天冬氨酰蛋白酶 (Sap) 活性。综上所述，我们的结果表明，白色-灰色-不透明三稳态转变的生物学特征和调节机制在两种致病性念珠菌属中很大程度上是保守的。
ABSTRACt Candida dubliniensis is closely related to Candida albicans, a major causative agent of candidiasis, and is primarily associated with oral colonization and infection in human immunodeficiency virus (HIV)-positive patients. Despite the high similarity of genomic and phenotypic features between the 2 species, C. dubliniensis is much less virulent and less prevalent than C. albicans. The ability to change morphological phenotypes is a striking feature of Candida species and is linked to virulence. In this study, we report a novel phenotype, the gray phenotype, in C. dubliniensis. Together with the previously reported white and opaque cell types, the gray phenotype forms a tristable phenotypic switching system in C. dubliniensis that is similar to the white-gray-opaque tristable switching system in C. albicans. Gray cells of C. dubliniensis are similar to their counterparts in C. albicans in terms of several biological aspects including cellular morphology, mating competence, and genetic regulatory mechanisms. However, the gray phenotypes of the 2 species have some distinguishing features. For example, the secreted aspartyl protease (Sap) activity is induced by bovine serum albumin (BSA) in gray cells of C. albicans, but not in gray cells of C. dubliniensis. Taken together, our results demonstrate that the biological features and regulatory mechanisms of white-gray-opaque tristable transitions are largely conserved in the 2 pathogenic Candida species.
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影响因子：
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