Identification of two polysaccharides from Prunella vulgaris L. and evaluation on their anti-lung adenocarcinoma activity.
Identification of two polysaccharides from Prunella vulgaris L. and evaluation on their anti-lung adenocarcinoma activity.
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DOI：
10.3390/molecules15085093
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发表时间：
2010-07-27
期刊：
影响因子：
--
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Feng L;Jia XB;Shi F;Chen Y
夏枯草[专业]植(PV)中药用于肿瘤治疗已有数百年的历史，但对具有多种生物活性的PV多糖提取物及其对肺癌的预防和抗肿瘤作用的系统研究尚未见报道。本研究从PV的水提液中分离得到两种多糖（P31和P32），并通过乙醇沉淀和DEAE-52凝胶过滤层析进行纯化。用气相色谱法分析了多糖P32的主要单糖组成。结果表明，多糖P32由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成，其摩尔比分别为3.46：49.32：58.91：0.43：2.64：3.11。为了研究多糖P32的抗肺腺癌活性和免疫调节作用，建立了C57 BL/6小鼠Lewis肺癌（LLC）模型。结果表明，PV多糖具有抗肺癌活性，并能提高荷瘤小鼠的胸腺指数和脾脏指数，提示其可能具有免疫调节作用。
Prunella vulgaris L. (PV) has been used for tumor therapy in Traditional Chinese Medicine for centuries, however, systematic research on extracted PV polysaccharides believed to possess various biological activities, as well as their preventive and anti-tumor effects on lung cancer has not been reported. In this study, two polysaccharides (P31 and P32) were isolated from the aqueous extract of PV and purified through ethanol precipitation, followed by deproteination using DEAE-52 gel-filtration chromatography. The main monosaccharide composition of polysaccharide P32 was analyzed by GC. It was found that polysaccharide P32 consisted of rhamnose, arabinose, xylose, mannose, glucose and galactose in a molar ratio of 3.46:49.32:58.91:0.43:2.64: 3.11, respectively. In order to evaluate polysaccharide P32’s anti-lung adenocarcinoma activities and immunomodulation effects, a C57BL/6 mouse-Lewis lung carcinoma (LLC) model was established and investigated. Our results showed that polysaccharides of PV had anti-lung cancer activity and could increase the thymus index and the spleen index in tumor-bearing mice, suggesting possible immunomodulation effects.
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影响因子：
4.3
作者：
Han, Eun Hee;Choi, Jae Ho;Jeong, Hye Gwang
通讯作者：
Jeong, Hye Gwang
影响因子：
7.2
作者：
Psotová, J;Kolár, M;Ulrichová, J
通讯作者：
Ulrichová, J
影响因子：
7.6
作者：
TABBA, HD;CHANG, RS;SMITH, KM
通讯作者：
SMITH, KM
DOI：
10.3390/molecules13102408
发表时间：
2008-10-01
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Xu DJ;Xia Q;Wang JJ;Wang PP
通讯作者：
Wang PP
影响因子：
3.3
作者：
Chen, Yi;Xie, Ming-Yong;Li, Chang
通讯作者：
Li, Chang