Hybrid origin of Asian aspermic Fasciola flukes is confirmed by analyzing two single-copy genes, pepck and pold.
Hybrid origin of Asian aspermic Fasciola flukes is confirmed by analyzing two single-copy genes, pepck and pold.
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DOI：
10.1292/jvms.17-0406
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发表时间：
2018-01-27
期刊：
影响因子：
--
通讯作者：
Itagaki T
中科院分区：
文献类型：
--
作者：
Hayashi K;Ichikawa-Seki M;Mohanta UK;Shoriki T;Chaichanasak P;Itagaki T
核基因标记，磷酸烯醇丙酮酸羧激酶（pepck）和DNA聚合酶δ（pold），已被开发用于精确区分片形吸虫，而不是内部转录间隔区1。在这项研究中，这两个基因的730片形吸虫来自8个亚洲国家进行了分析。结果进行了比较，他们的线粒体NADH脱氢酶亚基1（nad 1）的谱系获得一个明确的证据的杂交起源的无精子片吸虫。所有被归类为无精子nad 1谱系的吸虫都具有F. hepatica和F. pepck和/或pold中的紫茎泽兰（混合型）。这些研究结果提供了明确的证据，无精子片形吸虫血统的混合起源，并建议“无精子片形吸虫”应被称为“杂交片形吸虫”。
Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1. In this study, these two genes of 730 Fasciola flukes from eight Asian countries were analyzed. The results were compared with their mitochondrial NADH dehydrogenase subunit 1 (nad1) lineages for obtaining a definitive evidence of the hybrid origin of aspermic Fasciola flukes. All the flukes categorized into the aspermic nad1 lineages possessed both the fragment patterns of F. hepatica and F. gigantica (mixed types) in pepck and/or pold. These findings provide clear evidence for the hybrid origin of aspermic Fasciola lineages and suggest that “aspermic Fasciola flukes” should hereafter be called “hybrid Fasciola flukes”.
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