Chronic wasting disease prions are not transmissible to transgenic mice overexpressing human prion protein.
Chronic wasting disease prions are not transmissible to transgenic mice overexpressing human prion protein.
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慢性消耗性疾病朊病毒不会传染给过度表达人朊病毒蛋白的转基因小鼠。
DOI：
10.1099/vir.0.024380-0
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Collinge J
中科院分区：
文献类型：
--
作者：
Sandberg MK;Al-Doujaily H;Sigurdson CJ;Glatzel M;O'Malley C;Powell C;Asante EA;Linehan JM;Brandner S;Wadsworth JD;Collinge J
慢性消耗性疾病(CWD)是一种普恩病毒疾病，影响自由放牧和圈养的鹿群，包括骡鹿、白尾鹿、落基山脉麋鹿和驼鹿。据报道，美国14个州、加拿大两个省和韩国都有CWD感染宫颈的报告。在北美，狩猎鹿鹿是一项受欢迎的运动，通过饮食传播慢性萎缩性脑炎病毒的人畜共患病的可能性尤其令人担忧。为了研究慢性萎缩性胃病(CWD)病毒对公众健康的潜在危害，我们研究了CWD感染的梅花鹿的脑和脊髓内接种PrP是否会将Prion感染传递给带有甲硫氨酸或Valine多态残基129位的高表达人类Pron蛋白的转基因小鼠。这些转基因小鼠已被广泛用于人和动物Pron疾病的传播研究，并对BSE和vCJD Prion敏感，从而可以与CWD进行比较。在这里，我们证明了这些小鼠对骡鹿CWD普恩病毒的感染完全具有抵抗力，并辩称与这种普恩病毒株/宿主组合相关的传播障碍比观察到的经典疯牛病普恩病毒更大。然而，CWD可能是由多个普恩病毒株引起的。将需要进一步的研究，以评估不同的鹿普恩病毒株的传播特性，因为它们的特征。
Chronic wasting disease (CWD) is a prion disease that affects free-ranging and captive cervids, including mule deer, white-tailed deer, Rocky Mountain elk and moose. CWD-infected cervids have been reported in 14 USA states, two Canadian provinces and in South Korea. The possibility of a zoonotic transmission of CWD prions via diet is of particular concern in North America where hunting of cervids is a popular sport. To investigate the potential public health risks posed by CWD prions, we have investigated whether intracerebral inoculation of brain and spinal cord from CWD-infected mule deer transmits prion infection to transgenic mice overexpressing human prion protein with methionine or valine at polymorphic residue 129. These transgenic mice have been utilized in extensive transmission studies of human and animal prion disease and are susceptible to BSE and vCJD prions, allowing comparison with CWD. Here, we show that these mice proved entirely resistant to infection with mule deer CWD prions arguing that the transmission barrier associated with this prion strain/host combination is greater than that observed with classical BSE prions. However, it is possible that CWD may be caused by multiple prion strains. Further studies will be required to evaluate the transmission properties of distinct cervid prion strains as they are characterized.
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影响因子：
5.4
作者：
Heisey, Dennis M.;Mickelsen, Natalie A.;Barr, Daniel J.
通讯作者：
Barr, Daniel J.
DOI：
10.1073/pnas.0604292103
发表时间：
2006-07-11
影响因子：
11.1
作者：
Asante, Emmanuel A.;Linehan, Jacqueline M.;Collinge, John
通讯作者：
Collinge, John
影响因子：
--
作者：
Anderson, C. Alan;Bosque, Patrick;Tyler, Kenneth L.
通讯作者：
Tyler, Kenneth L.
影响因子：
--
作者：
Belay, ED;Gambetti, P;Nichols, CR
通讯作者：
Nichols, CR
DOI：
10.1080/15287390903084249
发表时间：
2009-01-01
影响因子：
2.6
作者：
Jennelle, Christopher S.;Samuel, Michael D.;Hoover, Edward A.
通讯作者：
Hoover, Edward A.