Affinity chromatography: A review of trends and developments over the past 50 years.

Affinity chromatography: A review of trends and developments over the past 50 years.
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DOI:
10.1016/j.jchromb.2020.122332
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发表时间:
2020-11-10
期刊:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子:
--
通讯作者:
Hage DS
Hage DS
中科院分区:
其他
文献类型:
--
作者:
Rodriguez EL;Poddar S;Iftekhar S;Suh K;Woolfork AG;Ovbude S;Pekarek A;Walters M;Lott S;Hage DS

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亲和色谱法领域采用生物相关试剂作为固定相,自1968年该方法的现代时代开始以来,已经出现了显着的增长。本文回顾了过去五十年来该技术的主要发展和趋势。首先讨论了这一领域的基本原理和历史。其次是概述了各种支持,固定化策略,并已在这一领域中使用的结合剂的类型。还考虑了亲和色谱的一般应用类型和使用领域。文献的调查是用来确定这些主题的主要趋势和重要领域的使用亲和色谱分离,分析,或表征的化学品和生物化学品。
The field of affinity chromatography, which employs a biologically-related agent as the stationary phase, has seen significant growth since the modern era of this method began in 1968. This review examines the major developments and trends that have occurred in this technique over the past five decades. The basic principles and history of this area are first discussed. This is followed by an overview of the various supports, immobilization strategies, and types of binding agents that have been used in this field. The general types of applications and fields of use that have appeared for affinity chromatography are also considered. A survey of the literature is used to identify major trends in these topics and important areas of use for affinity chromatography in the separation, analysis, or characterization of chemicals and biochemicals.
DOI: 10.1101/sqb.1968.033.01.033
发表时间: 1968-01-01
期刊: COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子: --
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