Proteomic Characterization of Senescent Laryngeal Adductor and Plantaris Hindlimb Muscles.
Proteomic Characterization of Senescent Laryngeal Adductor and Plantaris Hindlimb Muscles.
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DOI：
10.1002/lary.29683
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发表时间：
2022-01
期刊：
影响因子：
2.6
通讯作者：
Johnson, Aaron M.
中科院分区：
文献类型：
--
作者：
Shembel, Adrianna C.;Kanshin, Evgeny;Ueberheide, Beatrix;Johnson, Aaron M.
本研究的目的是1)比较喉和后肢肌肉的整体蛋白质表达，以及2)使用无标记的整体蛋白质组学方法研究老年和非老年肌肉中蛋白质表达的差异。对10只F344xBN F1雄性大鼠(老年和青年各5只)的喉内甲状肌和足底后肢肌进行了LC-MS/MS分析。对每种肌肉类型(喉部和四肢)和年龄组(老年和年轻肌肉)的蛋白质表达进行比较和路径丰富分析。通过LC-MS/MS分析，在肌肉类型和年龄组中发现了1000多种共同的蛋白质。在足底后肢的107个蛋白质和甲颧喉肌的19个蛋白质中，存在显著的年龄相关差异。生物信息学和浓缩分析表明，后肢和喉部的蛋白质差异可能与免疫和应激氧化还原反应以及RNA修复有关。喉和后肢骨骼肌之间的蛋白质表达有明显的差异。初步分析表明，这两组肌肉之间的差异可能与压力反应和修复机制有关。与年龄相关的变化，在甲颧骨似乎没有在足底明显。需要进一步的深入研究来阐明衰老如何影响喉部肌肉中的蛋白质表达。
The goals of this study were to 1) compare global protein expression in muscles of the larynx and hindlimb and 2) investigate differences in protein expression between aged and nonaged muscle using label-free global proteomic profiling methods. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis was performed on thyroarytenoid intrinsic laryngeal muscle and plantaris hindlimb muscle from 10 F344xBN F1 male rats (5 old and 5 young). Protein expression was compared and pathway enrichment analysis performed for each muscle type (larynx and limb) and age group (old and young muscle). Over 1,000 proteins were identified in common across both muscle types and age groups using LC-MS/MS analysis. Significant age-related differences were seen across 107 proteins in plantaris hindlimb and in 19 proteins in thyroarytenoid laryngeal muscle. Bioinformatic and enrichment analysis demonstrated protein differences between the hindlimb and larynx may relate to immune and stress redox responses and RNA repair. There are clear differences in protein expressions between the laryngeal and hindlimb skeletal muscles. Initial analysis suggests differences between the two muscle groups may relate to stress responses and repair mechanisms. Age-related changes in the thyroarytenoid appear to be less obvious than in the plantaris. Further in-depth study is needed to elucidate how aging affects protein expression in the laryngeal muscles.
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DOI：
10.1093/gerona/63.8.829
发表时间：
2008-08-01
影响因子：
5.1
作者：
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