Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1beta-stimulated fibroblast-like synoviocytes and in rat arthritis models.
Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1beta-stimulated fibroblast-like synoviocytes and in rat arthritis models.
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DOI：
10.1186/ar2662
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发表时间：
2009
影响因子：
4.9
通讯作者：
Kim KS
中科院分区：
文献类型：
--
作者：
Bang JS;Oh DH;Choi HM;Sur BJ;Lim SJ;Kim JY;Yang HI;Yoo MC;Hahm DH;Kim KS
本研究的目的是确定胡椒碱的抗炎、伤害和抗关节炎作用，胡椒碱是黑胡椒提取物中的活性酚类成分。研究了胡椒碱对类风湿性关节炎患者il - 1β刺激的成纤维细胞样滑膜细胞的体外抗炎活性。采用ELISA和RT-PCR检测各组大鼠血清il - 6、基质金属蛋白酶（MMPs）、环氧化酶2 （COX-2）、前列腺素E2 （PGE2）水平。采用卡拉胶致大鼠急性足痛和关节炎模型研究胡椒碱的镇痛和抗关节炎活性。前者通过爪压力测试来评估，后者通过测量吱吱声评分、爪体积和重量分布比来评估。大鼠口服胡椒碱20、100 mg/kg/d，连续8 d。在10 ~ 100 μg/ml浓度下，胡椒碱抑制il - 6和MMP13的表达，降低PGE2的生成，并呈剂量依赖性。特别是，即使在10 μg/ml的胡椒碱中，PGE2的产生也被显著抑制。在il -1 β处理的滑膜细胞中，胡椒碱能抑制活化蛋白1 （AP-1）向细胞核的迁移，而对核因子(NF)κB无抑制作用。在大鼠中，胡椒碱在第8天和第4天分别显著减轻了伤害性和关节炎症状。组织学染色显示胡椒碱能明显减少踝关节炎症区。这些结果表明胡椒碱在关节炎动物模型中具有抗炎、抗炎和抗关节炎作用。因此，胡椒碱作为治疗关节炎的药物或膳食补充剂应进一步研究。
The objective of this study was to determine the anti-inflammatory, nociceptive, and antiarthritic effects of piperine, the active phenolic component in black pepper extract. The in vitro anti-inflammatory activity of piperine was tested on interleukin 1β (IL1β)-stimulated fibroblast-like synoviocytes derived form patients with rheumatoid arthritis. The levels of IL6, matrix metalloproteinase (MMPs), cyclo-oxygenase 2 (COX-2), and prostaglandin E2 (PGE2) were investigated by ELISA and RT-PCR analysis. The analgesic and antiarthritic activities of piperine were investigated on rat models of carrageenan-induced acute paw pain and arthritis. The former were evaluated with a paw pressure test, and the latter by measuring the squeaking score, paw volume, and weight distribution ratio. Piperine was administrated orally to rats at 20 and 100 mg/kg/day for 8 days. Piperine inhibited the expression of IL6 and MMP13 and reduced the production of PGE2 in a dose dependant manner at concentrations of 10 to 100 μg/ml. In particular, the production of PGE2 was significantly inhibited even at 10 μg/ml of piperine. Piperine inhibited the migration of activator protein 1 (AP-1), but not nuclear factor (NF)κB, into the nucleus in IL1β-treated synoviocytes. In rats, piperine significantly reduced nociceptive and arthritic symptoms at days 8 and 4, respectively. Histological staining showed that piperine significantly reduced the inflammatory area in the ankle joints. These results suggest that piperine has anti-inflammatory, antinociceptive, and antiarthritic effects in an arthritis animal model. Thus, piperine should be further studied with regard to use either as a pharmaceutical or as a dietary supplement for the treatment of arthritis.
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影响因子：
3.4
作者：
Lee, Yeon-Ah;Kim, Jung Yeon;Yang, Hyung-In
通讯作者：
Yang, Hyung-In
影响因子：
7.4
作者：
Kwon, YB;Lee, JD;Lee, JH
通讯作者：
Lee, JH
牛磺酸氯胺差异抑制了白介素-1beta中的基质金属蛋白酶1和13合成刺激的成纤维细胞样细胞。
影响因子：
4.9
作者：
Kim, Kyoung Soo;Park, Eun Kyung;Ju, Seung Min;Jung, Hye-Sook;Bang, Jun Soo;Kim, Chaekyun;Lee, Yeon-Ah;Hong, Seung-Jae;Lee, Sang-Hoon;Yang, Hyung-In;Yoo, Myung Chul
通讯作者：
Yoo, Myung Chul
影响因子：
9.7
作者：
SINGH, J;REEN, RK;WIEBEL, FJ
通讯作者：
WIEBEL, FJ
影响因子：
7.2
作者：
Chrubasik, J. E.;Roufogalis, B. D.;Chrubasik, S.
通讯作者：
Chrubasik, S.