Pulmonary Protein Oxidation and Oxidative Stress Modulation by Lemna minor L. in Progressive Bleomycin-Induced Idiopathic Pulmonary Fibrosis.
Pulmonary Protein Oxidation and Oxidative Stress Modulation by Lemna minor L. in Progressive Bleomycin-Induced Idiopathic Pulmonary Fibrosis.
复制标题
DOI：
10.3390/antiox11030523
复制
发表时间：
2022-03-08
期刊：
影响因子：
--
通讯作者：
Nikolova G
中科院分区：
文献类型：
--
作者：
Karamalakova Y;Stefanov I;Georgieva E;Nikolova G
博来霉素（BLM）给药与多功能蛋白质炎症和特发性肺纤维化（IPF）的诱导有关。青萍提取物，自由漂浮的单子叶植物具有抗氧化和抗炎潜力。本研究的目的是检测L.微量提取物对Balb/c小鼠中BLM诱导的肺纤维化引起的肺蛋白氧化和氧化应激调节的影响。为此，检测了肺细胞中的蛋白质羰基含量、晚期糖基化终产物、氮氧蛋白氧化（5-MSL）和脂质过氧化（如MDA和ROS）。观察肺组织和血液中的组织学检查、胶原沉积以及IL-1β、IL-6和TNF的定量测定。腹膜内，BLM给药（0.069 U/mL; 0.29 U/kg b.w.）持续33天，在Balb/c小鼠中引起IPF诱导。肺综合治疗采用L.剂量120 mg/mL（0.187 mg/kg b.w.）时轻微。L.在肺组织中轻微的组织学改善BLM诱导的IPF。L.在肺羟脯氨酸、羰基化蛋白质、5-MSL-蛋白质氧化中诱导的BLM-改变被轻微显著调节（p < 0.05）。肺组织中抗氧化酶系统和非酶系统的氧化应激降低水平，L. minor. L.轻度降低肺组织和血浆中IL-1β、IL-6和TNF-α的表达。洛杉矶minor改善了BLM诱导的慢性炎症在特定肺蛋白氧化、脂质过氧化、ROS识别和细胞因子调节中的预防作用/防御反应，并且可能是一种良好的抗氧化剂、抗炎和抗纤维化替代或IPF预防，其发病机制涉及。
Bleomycin (BLM) administration is associated with multifunctional proteins inflammations and induction of idiopathic pulmonary fibrosis (IPF). Lemna minor L. extract, a free-floating monocot macrophyte possesses antioxidant and anti-inflammatory potential. The aim of the study was to examine the protective effect of L. minor extract on lung protein oxidation and oxidative stress modulation by BLM-induced pulmonary fibrosis in Balb/c mice. For this purpose, the protein carbonyl content, advanced glycation end product, nitroxide protein oxidation (5-MSL), and lipid peroxidation (as MDA and ROS), in lung cells were examined. The histological examinations, collagen deposition, and quantitative measurements of IL-1β, IL-6, and TNF in lung tissues and blood were investigated. Intraperitoneal, BLM administration (0.069 U/mL; 0.29 U/kg b.w.) for 33 days, caused IPF induction in Balb/c mice. Pulmonary combining therapy was administered with L. minor at dose 120 mg/mL (0.187 mg/kg b.w.). L. minor histologically ameliorated BLM induced IPF in lung tissues. L. minor significantly modulated (p < 0.05) BLM-alterations induced in lung hydroxyproline, carbonylated proteins, 5-MSL-protein oxidation. Oxidative stress decreased levels in antioxidant enzymatic and non-enzymatic systems in the lung were significantly regulated (p < 0.05) by L. minor. L. minor decreased the IL-1β, IL-6, and TNF-α expression in lung tissues and plasma. The L. minor improves the preventive effect/defense response in specific pulmonary protein oxidation, lipid peroxidation, ROS identifications, and cytokine modulation by BLM-induced chronic inflammations, and could be a good antioxidant, anti-inflammatory, and anti-fibrotic alternative or IPF prevention involved in their pathogenesis.
登录
查看更多内容
影响因子：
6.4
作者：
Cha SI;Chang CS;Kim EK;Lee JW;Matthay MA;Golden JA;Elicker BM;Jones K;Collard HR;Wolters PJ
通讯作者：
Wolters PJ
影响因子：
5.8
作者：
Fois AG;Paliogiannis P;Sotgia S;Mangoni AA;Zinellu E;Pirina P;Carru C;Zinellu A
通讯作者：
Zinellu A
DOI：
10.1513/pats.201203-023aw
发表时间：
2012-07-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Fernandez, Isis E;Eickelberg, Oliver
通讯作者：
Eickelberg, Oliver
影响因子：
6.1
作者：
Garbuzenko, E;Nagler, A;Levi-Schaffer, F
通讯作者：
Levi-Schaffer, F
Duckweeds：它们的利用，代谢产物和种植。
影响因子：
3.2
作者：
Baek G;Saeed M;Choi HK
通讯作者：
Choi HK