Reconstitution of Protein Dynamics Involved in Bacterial Cell Division.

Reconstitution of Protein Dynamics Involved in Bacterial Cell Division.
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细菌细胞分裂中涉及的蛋白质动力学的重建

DOI:
10.1007/978-3-319-53047-5_15
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发表时间:
2017
影响因子:
--
通讯作者:
Zieske K
Zieske K
中科院分区:
--
文献类型:
--
作者:
Loose M;Zieske K

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即使是像细菌这样的简单细胞,也有精确调节的细胞解剖结构,这使它们能够生长,分裂并对内部或外部线索做出高度逼真的反应。在原核细胞中,空间和时间的细胞内组织是如何在局部相互作用蛋白质的基础上实现和维持的,这在很大程度上仍然是一个谜。大量的生化分析与纯化的成分和体内实验帮助我们接近关键的细胞过程从两个相反的两端,在最小和最大的复杂性。然而,为了理解细胞现象是如何出现的,而不仅仅是其部分的总和,我们必须从下而上一步一步地组装细胞子系统。在这里,我们回顾了最近与细菌细胞分裂机制的蛋白质的vitroreconstruction实验,并说明它们如何有助于阐明构成时空秩序和调节细胞分裂的基本细胞机制。
Even simple cells like bacteria have precisely regulated cellular anatomies, which allow them to grow, divide and to respond to internal or external cues with high fidelity. How spatial and temporal intracellular organization in prokaryotic cells is achieved and maintained on the basis of locally interacting proteins still remains largely a mystery. Bulk biochemical assays with purified components andin vivoexperiments help us to approach key cellular processes from two opposite ends, in terms of minimal and maximal complexity. However, to understand how cellular phenomena emerge, that are more than the sum of their parts, we have to assemble cellular subsystems step by step from the bottom up. Here, we review recentin vitroreconstitution experiments with proteins of the bacterial cell division machinery and illustrate how they help to shed light on fundamental cellular mechanisms that constitute spatiotemporal order and regulate cell division.
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