Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia.
Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia.
复制标题
DOI：
10.1371/journal.pone.0028769
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Zgur-Bertok D
中科院分区：
文献类型：
--
作者：
Budič M;Rijavec M;Petkovšek Z;Zgur-Bertok D
细菌素是抗菌肽，通常对与生产者密切相关的细菌具有活性。大肠杆菌产生两种类型的细菌素：大肠菌素和小菌素。针对来自泌尿道源性菌血症患者的大肠杆菌菌株，评估了分离的大肠杆菌素 E1、E6、E7、K 和 M 的体外功效。大肠杆菌素 E7 最为有效，因为只有 13% 的测试菌株具有耐药性。另一方面，32%、33%、43%和53%的测试菌株表现出对大肠杆菌素E6、K、M和E1的抗性。此外，在液体肉汤中跟踪单个大肠菌素E1、E6、E7、K和M以及大肠菌素K、M、E7和E1、E6、E7、K、M的组合的抑制活性24小时。治疗 9 小时后，对单个大肠杆菌素产生了耐药性。相反，没有观察到对5种大肠菌素的联合作用产生抗性。通过 PCR 对来自菌血症患者的 105 株大肠杆菌菌株进行了筛选，以确定是否存在 5 种大肠菌素和 7 种小菌素。 66% 的菌株编码至少一种细菌素，43% 编码一种或多种大肠菌素，54% 编码一种或多种小菌素。研究发现小菌素与毒素、铁载体、粘附素以及含有 Toll/Interleukin-1 受体结构域的蛋白质共存，参与抑制先天免疫，并且在来自非免疫功能低下患者的菌株中明显更为普遍。此外，与多重耐药菌株相比，小菌素在非多重耐药菌株中非常普遍。我们的结果表明，小菌素有助于大肠杆菌的毒力，从而引发尿路源性菌血症。
Bacteriocins are antimicrobial peptides generally active against bacteria closely related to the producer. Escherichia coli produces two types of bacteriocins, colicins and microcins. The in vitro efficacy of isolated colicins E1, E6, E7, K and M, was assessed against Escherichia coli strains from patients with bacteraemia of urinary tract origin. Colicin E7 was most effective, as only 13% of the tested strains were resistant. On the other hand, 32%, 33%, 43% and 53% of the tested strains exhibited resistance to colicins E6, K, M and E1. Moreover, the inhibitory activity of individual colicins E1, E6, E7, K and M and combinations of colicins K, M, E7 and E1, E6, E7, K, M were followed in liquid broth for 24 hours. Resistance against individual colicins developed after 9 hours of treatment. On the contrary, resistance development against the combined action of 5 colicins was not observed. One hundred and five E. coli strains from patients with bacteraemia were screened by PCR for the presence of 5 colicins and 7 microcins. Sixty-six percent of the strains encoded at least one bacteriocin, 43% one or more colicins, and 54% one or more microcins. Microcins were found to co-occur with toxins, siderophores, adhesins and with the Toll/Interleukin-1 receptor domain-containing protein involved in suppression of innate immunity, and were significantly more prevalent among strains from non-immunocompromised patients. In addition, microcins were highly prevalent among non-multidrug-resistant strains compared to multidrug-resistant strains. Our results indicate that microcins contribute to virulence of E. coli instigating bacteraemia of urinary tract origin.
登录
查看更多内容
影响因子：
64.8
作者：
Kirkup, BC;Riley, MA
通讯作者：
Riley, MA
影响因子：
6.4
作者：
Johnson, JR;Kuskowski, MA;Vila, J
通讯作者：
Vila, J
影响因子：
2.8
作者：
Gordon, David M.;O'Brien, Claire L.
通讯作者：
O'Brien, Claire L.
影响因子：
3.1
作者：
JOHNSON, JR;MOSELEY, SL;STAMM, WE
通讯作者：
STAMM, WE
影响因子：
2.8
作者：
BRADLEY, DE;HOWARD, SP;LIOR, H
通讯作者：
LIOR, H